Analytical Data
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Gene name
EBP1
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简介
EBP1 protein participates in the signal transduction pathway regulated by ERBB3 and is related to growth regulation. As an androgen receptor (AR) corepressor, it is regulated by the ERBB3 ligand neregulin-1/heregulin (HRG). EBP1 Protein, Human (His) is the recombinant human-derived EBP1 protein, expressed by E. coli , with N-His labeled tag.
- Application
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Alternative Names
Proliferation-associated protein 2G4; hG4-1; ErbB3-binding protein 1; PA2G4
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Species
Human
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Source
E. coli
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Tag
N-His
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
Q9UQ80-1
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Expression Region
S2-D394
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AA Sequence
SGEDEQQEQTIAEDLVVTKYKMGGDIANRVLRSLVEASSSGVSVLSLCEKGDAMIMEETGKIFKKEKEMKKGIAFPTSISVNNCVCHFSPLKSDQDYILKEGDLVKIDLGVHVDGFIANVAHTFVVDVAQGTQVTGRKADVIKAAHLCAEAALRLVKPGNQNTQVTEAWNKVAHSFNCTPIEGMLSHQLKQHVIDGEKTIIQNPTDQQKKDHEKAEFEVHEVYAVDVLVSSGEGKAKDAGQRTTIYKRDPSKQYGLKMKTSRAFFSEVERRFDAMPFTLRAFEDEKKARMGVVECAKHELLQPFNVLYEKEGEFVAQFKFTVLLMPNGPMRITSGPFEPDLYKSEMEVQDAELKALLQSSASRKTQKKKKKKASKTAENATSGETLEENEAGD
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Protein Length
Full Length of Mature Protein
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Molecular Weight
45 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
EBP1 (Estrogen receptor binding protein 1) is a member of the EBP family, known for its role in various cellular processes, including cellular proliferation, differentiation, and response to stress. The research background of EBP1 recombinant protein stems from its critical involvement in estrogen signaling pathways and its potential implications in cancer biology, particularly in breast cancer, where estrogen plays a pivotal role in tumor progression. Studies have indicated that EBP1 interacts with estrogen receptors, influencing gene expression and possibly modulating cellular responses to hormonal treatments. Furthermore, as a protein that exhibits anti-apoptotic properties, EBP1 has garnered attention for its potential to be a therapeutic target. Researchers aim to produce recombinant EBP1 to investigate its structural and functional characteristics, enabling a better understanding of its mechanism in estrogen-mediated signaling. Purified EBP1 protein can serve as a valuable tool in biochemical assays, helping elucidate its interaction with other proteins and its role in cellular pathways. Additionally, exploring the post-translational modifications and interactions of EBP1 in various cellular contexts may provide insights into its function and potential as a biomarker or therapeutic agent in hormone-related cancers. Thus, studying EBP1 recombinant protein has become a crucial area of research, contributing to the understanding of complex regulatory networks in cancer biology and paving the way for innovative therapeutic strategies.
