Analytical Data
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Gene name
MLL3-WDR5-Ash2L-RbBP5-DPY30
- Application
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Alternative Names
HALR; KIAA1506; MLL3; WDR5; Ash2L; RbBP5; DPY30
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Species
Human
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Source
E. coli
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Tag
N-6*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q8NEZ4-1 (F4689-N4911)&P61964 (A2-C334)&Q9UBL3-3 (D2-P534)&Q15291-1 (N2-L538)&Q9C005
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Expression Region
Q8NEZ4-1 (F4689-N4911)&P61964 (A2-C334)&Q9UBL3-3 (D2-P534)&Q15291-1 (N2-L538)&Q9C005 (E2-N99)
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Molecular Weight
72-75 kDa (RbBP5) & 60-70 kDa (Ash2L) & 37-40 kDa (WDR5) & 27 kDa (MLL3) & 16-18 kDa (WDR5)
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
MLL3-WDR5-Ash2L-RbBP5-DPY30 complex is pivotal in the field of epigenetics, particularly in the regulation of gene expression through histone methylation. This multi-protein complex, part of the MLL (Mixed-Lineage Leukemia) family, functions as a histone methyltransferase, specifically promoting the trimethylation of histone H3 at lysine 4 (H3K4me3), a hallmark of active transcription. Dysregulation of the MLL3 complex has been implicated in various cancers and developmental disorders, highlighting its importance in maintaining normal cellular functions. The study of the recombined proteins within this complex is essential for understanding its structural and functional mechanisms. Researchers aim to elucidate how the interplay between these components affects histone modification and gene activity. Furthermore, insights into the structural biology and enzymatic mechanisms of MLL3-WDR5-Ash2L-RbBP5-DPY30 are critical for identifying potential therapeutic targets for diseases linked to epigenetic modifications. By investigating the dynamics and interactions of each protein within this complex, scientists hope to unravel the intricacies of chromatin regulation, paving the way for innovative strategies in cancer treatment and regenerative medicine. Ultimately, understanding this complex will contribute significantly to the broader field of epigenetics and its implications in human health.
