Analytical Data
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基因名
CDK12-CCNK
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简介
The CDK12 protein is a cyclin-dependent kinase that critically phosphorylates the C-terminal domain of POLR2A, thereby regulating transcription elongation. It selectively targets "Ser-5" phosphorylated at "Ser-7" in the CTD repeat and can also target "Ser-2". CDK12-CCNK Protein, Human (696-1082, sf9, GST) is the recombinant human-derived CDK12-CCNK, expressed by Sf9 insect cells, with N-GST labeled tag.
- Application
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别名
CRK7; CRKRS; KIAA0904
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种属
Human
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表达系统
Baculovirus
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标签
N-GST
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9NYV4-1 (Q696-S1082)&O75909-3
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表达区间
Q9NYV4-1 (Q696-S1082)&O75909-3 (K2-R580)
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分子量
90-100 kDa (CCNK) & 70-75 kDa (CDK12)
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The CDK12-CCNK protein complex has garnered significant interest in the field of molecular biology and cancer research due to its roles in regulating transcription and RNA processing. CDK12, or cyclin-dependent kinase 12, is a member of the cyclin-dependent kinase family and is known to phosphorylate RNA polymerase II, thereby playing a crucial role in the transcriptional regulation of various genes, especially those involved in DNA damage response and repair. CCNK, or cyclin K, acts as a cyclin that partners with CDK12 to influence its activity and substrate specificity. Dysregulation of the CDK12-CCNK complex has been implicated in cancer, particularly in certain types of tumors where mutations or alterations in the expression of these proteins can lead to aberrant transcriptional processes. Recent studies have shown that the CDK12-CCNK complex also participates in the regulatory network that controls the expression of genes associated with cell cycle progression and apoptosis, making it a potential target for therapeutic intervention. Understanding the structure and function of the CDK12-CCNK complex, as well as its interaction with other cellular components, could provide insights into the molecular mechanisms underlying tumorigenesis and identify new avenues for cancer treatment, thus highlighting the importance of investigating recombinant proteins of this complex in both basic and applied research.
