Analytical Data
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Gene name
CDK12-CCNK
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简介
The CDK12 protein is a cyclin-dependent kinase that critically phosphorylates the C-terminal domain of POLR2A, thereby regulating transcription elongation. It selectively targets "Ser-5" phosphorylated at "Ser-7" in the CTD repeat and can also target "Ser-2". CDK12-CCNK Protein, Human (696-1082, sf9, GST) is the recombinant human-derived CDK12-CCNK, expressed by Sf9 insect cells, with N-GST labeled tag.
- Application
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Alternative Names
CRK7; CRKRS; KIAA0904
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Species
Human
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Source
Baculovirus
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Tag
N-GST
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9NYV4-1 (Q696-S1082)&O75909-3
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Expression Region
Q9NYV4-1 (Q696-S1082)&O75909-3 (K2-R580)
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Molecular Weight
90-100 kDa (CCNK) & 70-75 kDa (CDK12)
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The CDK12-CCNK protein complex has garnered significant interest in the field of molecular biology and cancer research due to its roles in regulating transcription and RNA processing. CDK12, or cyclin-dependent kinase 12, is a member of the cyclin-dependent kinase family and is known to phosphorylate RNA polymerase II, thereby playing a crucial role in the transcriptional regulation of various genes, especially those involved in DNA damage response and repair. CCNK, or cyclin K, acts as a cyclin that partners with CDK12 to influence its activity and substrate specificity. Dysregulation of the CDK12-CCNK complex has been implicated in cancer, particularly in certain types of tumors where mutations or alterations in the expression of these proteins can lead to aberrant transcriptional processes. Recent studies have shown that the CDK12-CCNK complex also participates in the regulatory network that controls the expression of genes associated with cell cycle progression and apoptosis, making it a potential target for therapeutic intervention. Understanding the structure and function of the CDK12-CCNK complex, as well as its interaction with other cellular components, could provide insights into the molecular mechanisms underlying tumorigenesis and identify new avenues for cancer treatment, thus highlighting the importance of investigating recombinant proteins of this complex in both basic and applied research.
