Analytical Data
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Gene name
CRIPT
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简介
CRIPT is a key member of the small spliceosome and plays a crucial role in U12-type intron splicing, contributing to the complexity of RNA splicing. CRIPT Protein, Human (His) is the recombinant human-derived CRIPT protein, expressed by E. coli , with N-His labeled tag.
- Application
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Alternative Names
Cysteine-rich PDZ-binding protein; CRIPT; HSPC139
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Species
Human
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Source
E. coli
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9P021
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Expression Region
M1-V101
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Protein Length
Full Length
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Molecular Weight
14 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CRIPT (Cytosolic RNA-binding Protein Interacting Protein) is an emerging player in cellular biology, particularly in the context of RNA metabolism and post-transcriptional regulation. It is known to interact with various RNA-binding proteins, thereby influencing mRNA stability, localization, and translation. The study of CRIPT and its recombinant protein forms has gained attention due to its potential roles in critical biological processes such as cell proliferation, differentiation, and stress response. Researchers are interested in understanding how CRIPT interacts with different cellular pathways, especially in relation to diseases such as cancer, where dysregulation of RNA-binding proteins can severely alter gene expression profiles. By developing CRIPT recombinant proteins, scientists aim to elucidate the functional mechanisms of CRIPT and its interactions within the cell, thereby providing insights into its role as a regulatory component in RNA processing and cellular homeostasis. This research is crucial for identifying new therapeutic targets and advancing our understanding of the molecular underpinnings of diseases linked to RNA metabolism dysregulation.
