Analytical Data
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Gene name
MME
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简介
MME proteins exhibit thermolysin-like specificity and primarily degrade polypeptides of up to 30 amino acids. Crucially, it plays a key role in cleaving the Gly-Phe bond to degrade opioid peptides, including Met-enkephalin and Leu-enkephalin. MME Protein, Human (HEK293) is the recombinant human-derived MME protein, expressed by HEK293 , with tag free.
- Application
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Alternative Names
Neprilysin; Atriopeptidase; CALLA; NEP; SFE; CD10; MME; EPN
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Species
Human
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Source
HEK293
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Tag
Tag Free
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
P08473
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Expression Region
Y52-W750
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AA Sequence
YDDGICKSSDCIKSAARLIQNMDATTEPCTDFFKYACGGWLKRNVIPETSSRYGNFDILRDELEVVLKDVLQEPKTEDIVAVQKAKALYRSCINESAIDSRGGEPLLKLLPDIYGWPVATENWEQKYGASWTAEKAIAQLNSKYGKKVLINLFVGTDDKNSVNHVIHIDQPRLGLPSRDYYECTGIYKEACTAYVDFMISVARLIRQEERLPIDENQLALEMNKVMELEKEIANATAKPEDRNDPMLLYNKMTLAQIQNNFSLEINGKPFSWLNFTNEIMSTVNISITNEEDVVVYAPEYLTKLKPILTKYSARDLQNLMSWRFIMDLVSSLSRTYKESRNAFRKALYGTTSETATWRRCANYVNGNMENAVGRLYVEAAFAGESKHVVEDLIAQIREVFIQTLDDLTWMDAETKKRAEEKALAIKERIGYPDDIVSNDNKLNNEYLELNYKEDEYFENIIQNLKFSQSKQLKKLREKVDKDEWISGAAVVNAFYSSGRNQIVFPAGILQPPFFSAQQSNSLNYGGIGMVIGHEITHGFDDNGRNFNKDGDLVDWWTQQSASNFKEQSQCMVYQYGNFSWDLAGGQHLNGINTLGENIADNGGLGQAYRAYQNYIKKNGEEKLLPGLDLNHKQLFFLNFAQVWCGTYRPEYAVNSIKTDVHSPGNFRIIGTLQNSAEFSEAFHCRKNSYMNPEKKCRVW
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Protein Length
Extracellular Domain
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Molecular Weight
80-105 kDa.
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The study of MME (membrane metalloendopeptidase), also known as neprilysin, is vital due to its significant role in maintaining various physiological functions and its implications in several pathological conditions. MME is a zinc-dependent metalloprotease that hydrolyzes a broad range of bioactive peptides, including neuropeptides and vasoactive peptides, thereby influencing processes such as blood pressure regulation, neurodegeneration, and inflammation. Its activity is particularly critical in the degradation of amyloid-beta peptides, linking it to Alzheimer’s disease pathology. Researchers have focused on understanding the structural and functional properties of MME to develop potential therapeutic strategies. Given the enzyme’s involvement in neurodegenerative disorders, selective inhibitors of MME have emerged as potential candidates for enhancing cognitive function and mitigating disease progression. Additionally, MME's expression levels and activity are associated with various cancers, further expanding the scope of its research as a biomarker or therapeutic target. Efforts are also directed toward elucidating the regulatory mechanisms governing MME expression and activity, which could inform strategies for modulating its function in clinical settings. Overall, the multifaceted role of MME in health and disease underscores the importance of ongoing research to exploit its potential in therapeutic interventions.
