Analytical Data
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Gene name
HN1
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简介
HN1 protein negatively regulates AKT-mediated GSK3B signaling and induces the phosphorylation and degradation of CTNNB1 at “Ser-33”. This effect inhibits the “Ser-9” phosphorylation of GSK3B, inhibits the activity of APC:CTNNB1:GSK3B complex and blocks its interaction with CDH1. HN1 Protein, Mouse (His) is the recombinant mouse-derived HN1 protein, expressed by E. coli , with N-His labeled tag.
- Application
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Alternative Names
Jupiter microtubule associated homolog 1; Jpt1; Hn1
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Species
Mouse
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Source
E. coli
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P97825
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Expression Region
M1-G154
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Protein Length
Full Length
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Molecular Weight
24 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The research on HN1 recombinant proteins has gained significant attention due to the rising concerns surrounding pandemic influenza strains and their potential threat to global health. HN1 refers to the hemagglutinin (HA) and neuraminidase (NA) proteins found on the surface of the H1N1 influenza virus, which are critical for the virus's ability to infect host cells and evade the immune response. By using recombinant DNA technology, scientists can produce these proteins in the laboratory, enabling the exploration of their structure, function, and interactions. This research facilitates the development of more effective vaccines and antiviral therapies, particularly as traditional methods may not keep pace with the rapid mutations of the virus. Understanding the immunogenic properties of HN1 recombinant proteins also aids in the identification of potential biomarkers for disease progression and vaccine responsiveness. Additionally, insights gained from these studies contribute to the broader field of virology and enhanced pandemic preparedness strategies. As new variants emerge, the ability to quickly produce and test recombinant proteins becomes crucial in responding to influenza outbreaks and safeguarding public health.
