Analytical Data
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Gene name
FNDC4
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简介
The FNDC4 protein is an anti-inflammatory factor in the intestine and colon that regulates macrophages by downregulating pro-inflammatory gene expression and affecting phagocytosis. It does this by modulating key pathways associated with macrophage activation, in part through STAT3 activation and signaling. FNDC4 Protein, Human (HEK293, Fc) is the recombinant human-derived FNDC4 protein, expressed by HEK293 , with C-mFc labeled tag.
- Application
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Biological Activity
Measured in a cell proliferation assay using U87 cells. The ED50 for this effect is 63 ng/mL. Corresponding to a specific activity is 1.587×104 unit/mg. Measured in a cell proliferation assay using U87 cells. The ED50 for this effect is 63 ng/mL. Corresponding to a specific activity is 1.587×104 Unit/mg.
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Alternative Names
Fibronectin type III domain-containing protein 4; Fibronectin type III repeat-containing protein 1; FRCP1
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Species
Human
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Source
HEK293
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Tag
C-mFc
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
Q9H6D8
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Expression Region
D45-T167
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Protein Length
Extracellular Domain
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Molecular Weight
50-60 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
FNDC4 is a recently identified protein that has garnered significant attention in the field of metabolic research due to its potential role in energy regulation and obesity. This protein is a secreted factor derived from the FNDC family, traditionally associated with cellular adhesion and signaling processes. Recent studies have revealed FNDC4's involvement in the modulation of brown adipose tissue (BAT) activity, ultimately influencing thermogenesis and energy expenditure. Preliminary research has indicated that FNDC4 may enhance the browning of white adipose tissue, promoting the conversion of white fat into thermogenic, calorie-burning brown fat. Additionally, FNDC4 is believed to be linked with insulin sensitivity and could play a crucial role in mitigating weight gain and metabolic disorders. The growing prevalence of obesity and its associated health complications underscore the urgent need for novel therapeutic targets, making FNDC4 a promising candidate for further investigation. Researchers are now focused on characterizing the biochemical mechanisms by which FNDC4 functions, particularly how it interacts with various metabolic signaling pathways. Understanding FNDC4's role could lead to innovative strategies for combating obesity and its related diseases, thus improving public health outcomes. The exploration of FNDC4, through the development of recombinant protein for in vitro and in vivo studies, holds the potential to uncover new insights into metabolic regulation and offers hope for the development of effective interventions in obesity-related disorders.
