Analytical Data
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Gene name
CDK12-CCNT1
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简介
The CDK12 protein is a cyclin-dependent kinase that critically phosphorylates the C-terminal domain of POLR2A, thereby regulating transcription elongation. It selectively targets "Ser-5" phosphorylated at "Ser-7" in the CTD repeat and can also target "Ser-2". CDK12-CCNT1 Protein, Human (sf9, GST, Flag, His) is the recombinant human-derived CDK12-CCNT1, expressed by Sf9 insect cells, with N-His, N-GST, N-Flag labeled tag.
- Application
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Alternative Names
CDK12; CCNK; Cyclin-dependent kinase 12; Cdc2-related kinase; arginine/serine-rich; CrkRS; Cell division cycle 2-related protein kinase 7; CDC2-related protein kinase 7; Cell division protein kinase 12; hCDK12; Cyclin-K
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Species
Human
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Source
Baculovirus
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Tag
N-His;N-GST;N-Flag
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9NYV4-1 (Q696-S1082)&O60563-1
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Expression Region
Q9NYV4-1 (Q696-S1082)&O60563-1 (M1-R259)
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CDK12-CCNT1 recombinant protein has garnered attention in recent years due to its pivotal role in transcriptional regulation and its implications in cancer biology. CDK12 (Cyclin-Dependent Kinase 12) is a cyclin-dependent kinase that plays a crucial role in RNA polymerase II phosphorylation, thereby influencing gene expression and mRNA processing. Its interaction with CCNT1 (Cyclin C-Dependent Kinase 12 Partner 1) is essential for facilitating the recruitment of RNA processing factors, affecting the stability and translation of various transcripts, including those related to DNA damage response and tumorigenesis. Dysregulation of the CDK12-CCNT1 complex has been implicated in various cancers, particularly in cases with mutations in DNA repair pathways, thus making it a target of interest for therapeutic interventions. Understanding the molecular mechanisms governing CDK12-CCNT1 activity provides insights into oncogenic processes and helps identify potential biomarkers or treatment strategies. The production and characterization of CDK12-CCNT1 recombinant protein enable researchers to explore its biochemical properties, post-translational modifications, and interactions with other cellular components, paving the way for novel cancer therapies that could specifically target this pathway.
