Analytical Data
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Gene name
CDK3-CCNE2
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简介
CDK3 protein is a serine/threonine protein kinase that plays a key role in regulating the eukaryotic cell cycle, especially affecting the G0-G1 and G1-S transitions. It interacts with CCNC/cyclin-C during interphase and phosphorylates substrates such as histone H1, ATF1, RB1, and CABLES1. CDK3-CCNE2 Protein, Human (sf9, GST, Flag, His) is the recombinant human-derived CDK3-CCNE2, expressed by Sf9 insect cells, with N-His, N-GST, N-Flag labeled tag.
- Application
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Alternative Names
CDK3; CCNE1; Cyclin-dependent kinase 3; Cell division protein kinase 3; G1/S-specific cyclin-E1
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Species
Human
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Source
Baculovirus
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Tag
N-His;N-GST;N-Flag
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q00526 (M1-H305)&O96020-1
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Expression Region
Q00526 (M1-H305)&O96020-1 (S2-H404 )
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CDK3-CCNE2 recombinant protein has emerged as a significant focus of research due to its critical role in cell cycle regulation and potential implications in cancer biology. Cyclin-dependent kinase 3 (CDK3), a member of the cyclin-dependent kinase family, is involved in the phosphorylation of proteins that are essential for the progression of the cell cycle, particularly during the G1 phase. Cyclin E2 (CCNE2), a regulatory subunit, partners with CDK3 to form a complex that governs key transition points in the cell cycle, influencing cellular proliferation and tumorigenesis. Dysregulation of this pathway has been implicated in various malignancies, where abnormal expression or activity of CDK3 and CCNE2 can lead to uncontrolled cell division. The production of CDK3-CCNE2 recombinant protein aims to facilitate in-depth studies of their biochemical interactions, regulatory mechanisms, and effects on cellular processes. Understanding the functional dynamics of this complex may reveal novel insights into the molecular underpinnings of cancer and promote the identification of new therapeutic targets. Additionally, elucidating the structure and function of CDK3-CCNE2 can enhance our knowledge of cell cycle checkpoints and potentially aid in the development of strategies to modulate these pathways for therapeutic benefit. Thus, the investigation of CDK3-CCNE2 recombinant protein is essential for advancing cancer research and improving treatment options for patients with malignancies characterized by cell cycle dysregulation.
