Analytical Data
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Gene name
CDK3-CCNC
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简介
CDK3 protein is a serine/threonine protein kinase that plays a key role in regulating the eukaryotic cell cycle, especially affecting the G0-G1 and G1-S transitions. It interacts with CCNC/cyclin-C during interphase and phosphorylates substrates such as histone H1, ATF1, RB1, and CABLES1. CDK3-CCNC Protein, Human (sf9, GST, Flag, His) is the recombinant human-derived CDK3-CCNC, expressed by Sf9 insect cells, with N-Flag, N-His, N-GST labeled tag.
- Application
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Alternative Names
CDK3; CCNE1; Cyclin-dependent kinase 3; Cell division protein kinase 3; G1/S-specific cyclin-E1
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Species
Human
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Source
Baculovirus
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Tag
N-Flag;N-His;N-GST
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q00526 (M1-H305)&P24863-1
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Expression Region
Q00526 (M1-H305)&P24863-1 (M1-S283)
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CDK3-CCNC recombinant protein is a focus of research due to its pivotal role in cell cycle regulation and its implications in various biological processes, including cellular proliferation and differentiation. Cyclin-dependent kinase 3 (CDK3) is a member of the CDK family, which is crucial for controlling the progression of cells through the cell cycle by phosphorylating specific substrates. CCNC, or cyclin C, forms a complex with CDK3, enhancing its kinase activity and stability. This CDK3-CCNC complex is particularly important in the transition from the G1 to S phase of the cell cycle. Dysregulation of CDK3 and CCNC has been implicated in several cancers, making it a potential target for therapeutic interventions. Researchers aim to produce CDK3-CCNC recombinant proteins to study their structural and functional properties, investigate their interactions with other cellular proteins, and explore their roles in tumorigenesis. Furthermore, understanding the dynamics of the CDK3-CCNC interaction may provide insights into developing novel strategies for cancer treatment, as inhibiting this pathway could restore normal cell cycle control in cancer cells. Therefore, the creation and analysis of CDK3-CCNC recombinant proteins are essential for advancing our knowledge of cell cycle regulation and developing targeted therapies for cancer.
