Analytical Data
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Gene name
CDK2-CCNE1
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简介
CDK2 is an important serine/threonine protein kinase that plays a critical regulatory role in the cell cycle, especially during meiosis. It phosphorylates various substrates (CTNNB1, USP37, p53/TP53, NPM1, CDK7, RB1, BRCA2, MYC, NPAT, EZH2) and coordinates centrosome duplication and initiation of DNA synthesis at the G1-S transition. CDK2-CCNE1 Protein, Human (sf9, GST, His, Flag) is the recombinant human-derived CDK2-CCNE1, expressed by Sf9 insect cells, with N-His, N-GST, N-Flag labeled tag.
- Application
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Alternative Names
CDK2; cyclin-dependent kinase 2; p33 protein kinase; cell devision kinase 2; cdc2-related protein kinase; cell division protein kinase 2; p33(CDK2)
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Species
Human
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Source
Baculovirus
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Tag
N-His;N-GST;N-Flag
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P24941-1 (M1-L298)&P24864-1
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Expression Region
P24941-1 (M1-L298)&P24864-1 (M1-E410)
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Cyclin-dependent kinase 2 (CDK2) and cyclin E1 (CCNE1) play critical roles in cell cycle regulation, particularly in the transition from the G1 phase to the S phase. Dysregulation of the CDK2-CCNE1 complex has been implicated in various types of cancer, as it can lead to uncontrolled cell proliferation. Researchers have focused on characterizing the CDK2-CCNE1 recombinant protein to better understand its structural properties, functional mechanisms, and interactions with other cell cycle regulators. The study of this protein complex not only enhances our understanding of the cell cycle but also opens avenues for the development of targeted therapies that could inhibit its activity in cancerous cells. By employing techniques such as X-ray crystallography and biochemical assays, researchers aim to elucidate the molecular basis of CDK2-CCNE1 activity, identify potential inhibitors, and assess the role of post-translational modifications in its function. These insights are essential for designing novel anticancer strategies that could selectively disrupt the aberrations associated with CDK2 and CCNE1, ultimately contributing to more effective cancer treatments.
