Analytical Data
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Gene name
Neurotrophin-3
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简介
Neurotrophin-3 (NT-3) is crucial for promoting the survival of sensory neurons, specifically those linked to visceral and proprioceptive functions. Its role highlights NT-3's significance in maintaining and supporting the functionality of these specialized sensory cells, emphasizing its potential as a key regulator in the complex network governing essential physiological sensory processes. Neurotrophin-3 Protein, Human (HEK293) is the recombinant human-derived Neurotrophin-3 protein, expressed by HEK293 , with tag free.
- Application
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Biological Activity
1. Measured in a cell proliferation assay using BaF3 mouse pro-B cells transfected with TrkB. The ED50 for this effect is 36.23 ng/mL, corresponding to a specific activity is 2.760×104 units/mg. 2. Human TrkC immobilized on CM5 Chip can bind Human NT-3 with an affinity constant of 0.0523 nM as determined in a SPR assay. Measured in a cell proliferation assay using BaF3 mouse pro-B cells transfected with TrkB. The ED50 for this effect is 36.23 ng/mL, corresponding to a specific activity is 2.760×104 units/mg.
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Alternative Names
HDNF; MGC129711; Nerve growth factor 2; Neurotrophic factor; neurotrophin 3; neurotrophin-3; NGF2; NGF-2; NT3; NT-3; NTF3
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Species
Human
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Source
HEK293
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Tag
Tag Free
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Purity
Greater than 95% as determined by reducing SDS-PAGE.
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Uniprot
P20783
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Expression Region
Y139-T257
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Protein Length
Full Length of Mature Protein
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Molecular Weight
14 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Neurotrophin-3 (NT-3) is a member of the neurotrophin family, which includes crucial growth factors playing vital roles in the development, survival, and function of neurons. Discovered in the early 1990s, NT-3 facilitates neuronal differentiation and connectivity, making it essential for neurodevelopmental processes and the maintenance of neuronal health. Research has highlighted its involvement in several neurological disorders, including neurodegenerative diseases and peripheral neuropathies, suggesting that modulating NT-3 levels may offer therapeutic potential. Recombinant NT-3 proteins have been generated to study their biological effects in vitro and in vivo, enabling investigations into their mechanisms of action and potential applications in regenerative medicine. Advances in recombinant protein technology have improved the efficacy and stability of NT-3, facilitating detailed studies on its signaling pathways and interactions with neurotrophic receptors. Understanding NT-3's role in neuronal biology not only contributes to fundamental neuroscience but also paves the way for innovative treatment strategies targeting neurodegeneration and injury recovery. Overall, the exploration of recombinant NT-3 proteins continues to provide valuable insights into neurotrophic signaling and therapeutic opportunities for a range of neurological conditions.
