Analytical Data
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Gene name
AMPK gamma 1/beta 1/alpha 1 Heterotrimer
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简介
AMPK gamma 1 is the AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK) and is an important regulator of cellular energy metabolism. It is activated by low ATP levels, enhancing energy production while inhibiting biosynthetic processes and cell growth. AMPK gamma 1/beta 2/alpha 1 Heterotrimer Protein, Human (sf9, His-GST) is the recombinant human-derived AMPK gamma 1/beta 2/alpha 1 Heterotrimer protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. AMPK gamma 1/beta 2/alpha 1 Heterotrimer Protein, Human (sf9, His-GST), has molecular weight of ~35 & 37 & 95 KDa, respectively.
- Application
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Alternative Names
5'-AMP-activated protein kinase; AMPK; AMPK (α1, β2, γ1) Protein
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Species
Human
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Source
Baculovirus
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Tag
N-His;N-GST
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P54619 (M1-P331)&O43741 (M1-I272)&Q13131
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Expression Region
P54619 (M1-P331)&O43741 (M1-I272)&Q13131 (M1-Q559)
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Molecular Weight
35&37&95 kDa.
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
AMP-activated protein kinase (AMPK) is a vital energy sensor in cellular metabolism, composed of three subunits: alpha (α), beta (β), and gamma (γ). The heterotrimeric complex, specifically the AMPK gamma 1 (γ1), beta 1 (β1), and alpha 1 (α1) subunits, plays a crucial role in regulating energy homeostasis by activating catabolic pathways and inhibiting anabolic processes in response to cellular energy depletion. The research into AMPK's structure and function has intensified due to its implications in metabolic diseases, such as obesity, diabetes, and cancer. The ability to produce recombinant AMPK heterotrimers in vitro has provided valuable insights into the intricate mechanisms of AMPK signaling and its activation by AMP and other nucleotides. Understanding the specific interactions and regulatory mechanisms of the AMPK γ1/β1/α1 complex enhances our knowledge of metabolic regulation and may lead to novel therapeutic strategies targeting this essential kinase to combat metabolic disorders and improve overall health. Thus, studying the recombinant protein forms of AMPK subunits serves as a fundamental approach to elucidating their functional roles within the signaling pathways that govern energy metabolism.
