Analytical Data
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基因名
RIPK3
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简介
RIPK3 Protein, participates in programmed necrosis (necroptosis) and apoptosis, contributing to diverse cellular processes. RIPK3 Protein, Mouse (P.pastoris, His) is the recombinant mouse-derived RIPK3 protein, expressed by P. pastoris , with N-6*His labeled tag.
- Application
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别名
Ripk3; Rip3; Receptor-interacting serine/threonine-protein kinase 3; EC 2.7.11.1; RIP-like protein kinase 3; Receptor-interacting protein 3; RIP-3; mRIP3
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种属
Mouse
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表达系统
P. pastoris
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标签
N-6*His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9QZL0
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表达区间
M1-K486
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氨基酸序列
MSSVKLWPTGASAVPLVSREELKKLEFVGKGGFGVVFRAHHRTWNHDVAVKIVNSKKISWEVKAMVNLRNENVLLLLGVTEDLQWDFVSGQALVTRFMENGSLAGLLQPECPRPWPLLCRLLQEVVLGMCYLHSLDPPLLHRDLKPSNILLDPELHAKLADFGLSTFQGGSQSGSGSGSGSRDSGGTLAYLDPELLFKVNLKASKASDVYSFGILVWAVLAGREAELVDKTSLIRETVCDRQSRPPLTELPPGSPETPGLEKLKELMIHCWGSQSENRPSFQDCEPKTNEVYNLVKDKVDAAVSEVKHYLSQHRSSGRNLSAREPSQRGTEMDCPRETMVSKMLDRLHLEEPSGPVPGKCPERQAQDTSVGPATPARTSSDPVAGTPQIPHTLPFRGTTPGPVFTETPGPHPQRNQGDGRHGTPWYPWTPPNPMTGPPALVFNNCSEVQIGNYNSLVAPPRTTASSSAKYDQAQFGRGRGWQPFHK
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蛋白长度
Full Length
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分子量
64 kDa.The reducing (R) protein migrat es as 64 kDa in SDS-PAGE may be due to molecular structure of protein.
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Receptor-interacting protein kinase 3 (RIPK3) is a crucial mediator of programmed cell death pathways, particularly necroptosis, a form of regulated necrosis that has garnered significant attention in recent years due to its implications in various diseases including cancer, neurodegeneration, and inflammatory disorders. The study of RIPK3 is essential because of its role in cell fate determination under stress conditions, where it can promote inflammatory responses and tissue damage. Research has shown that RIPK3 interacts with various signaling molecules, influencing not only cell death but also survival pathways, thereby presenting it as a potential therapeutic target. The recombinant expression of RIPK3 protein in vitro allows for detailed characterization of its enzymatic activities, interaction partners, and regulatory mechanisms. Understanding the structure-function relationship of RIPK3 through recombinant techniques can provide insights into its role in necroptosis and other cellular processes, contributing to the development of targeted therapies that modulate RIPK3 activity for therapeutic benefit. As such, RIPK3 remains a focal point in the study of cell death mechanisms, and the generation of recombinant RIPK3 proteins is a pivotal step in elucidating its multifaceted functions within the context of cellular stress responses and disease pathology.
