Analytical Data
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Gene name
IBSP
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简介
The IBSP protein has a strong affinity for hydroxyapatite and plays a crucial role in mineralized matrix formation, indicating its overall nature in this regard. Its tight binding suggests an important role in cell-matrix interactions, particularly in promoting RGD-dependent cell attachment. IBSP Protein, Human (His-SUMO) is the recombinant human-derived IBSP protein, expressed by E. coli , with N-6*His, N-SUMO labeled tag.
- Application
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Alternative Names
BNSP; Bone sialoprotein 2; Bone sialoprotein II; BSP; BSP II; BSPII; Cell binding sialoprotein; Cell-binding sialoprotein; IBSP; Integrin binding sialoprotein; Integrin-binding sialoprotein; SIAL_HUMAN; SPII
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Species
Human
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Source
E. coli
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Tag
N-6*His;N-SUMO
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P21815
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Expression Region
A129-E281
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Protein Length
Partial
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Molecular Weight
40 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
IBSP (Integrin Binding Sialoglycoprotein) is a cell adhesion molecule that plays a pivotal role in bone biology and the regulation of mineralization. It is primarily expressed by osteoblasts and osteocytes, contributing to bone formation and remodelling through its interactions with integrins and other extracellular matrix components. Recent studies have highlighted the significance of IBSP in various physiological and pathological conditions, including bone diseases such as osteoporosis and metastatic cancer. The recombinant expression of IBSP proteins has become an essential tool for investigating its functional properties and biological mechanisms. By producing IBSP in a recombinant form, researchers can explore its structural characteristics, binding affinities, and interactions with cell surface receptors, facilitating a better understanding of its role in bone health and disease. Additionally, recombinant IBSP can be utilized in therapeutic applications, such as designing biomaterials for bone regeneration and enhancing osteogenic differentiation. Overall, the study of IBSP recombinant proteins provides valuable insights into the molecular underpinnings of bone biology and opens new avenues for developing targeted therapies for skeletal disorders.
