Analytical Data
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基因名
AKT1
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简介
The AKT1 protein is part of the AKT kinase family and is critical for metabolism, proliferation, survival, growth, and angiogenesis. It phosphorylates more than 100 substrates, coordinates insulin-induced SLC2A4/GLUT4 translocation, affects glucose uptake, and controls glycogen storage through inhibition of GSK3A/B. AKT1 Protein, Human (S473D, sf9, His, GST) is the recombinant human-derived AKT1 protein, expressed by Sf9 insect cells, with N-His and N-GST labeled tag.
- Application
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别名
RAC-alpha serine/threonine-protein kinase; PKB; RAC-PK-alpha; AKT1; RAC
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种属
Human
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表达系统
Baculovirus
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标签
N-His;N-GST
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P31749
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表达区间
S473D, M118-A480
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氨基酸序列
MDFRSGSPSDNSGAEEMEVSLAKPKHRVTMNEFEYLKLLGKGTFGKVILVKEKATGRYYAMKILKKEVIVAKDEVAHTLTENRVLQNSRHPFLTALKYSFQTHDRLCFVMEYANGGELFFHLSRERVFSEDRARFYGAEIVSALDYLHSEKNVVYRDLKLENLMLDKDGHIKITDFGLCKEGIKDGATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVMYEMMCGRLPFYNQDHEKLFELILMEEIRFPRTLGPEAKSLLSGLLKKDPKQRLGGGSEDAKEIMQHRFFAGIVWQHVYEKKLSPPFKPQVTSETDTRYFDEEFTAQMITITPPDQDDSMECVDSERRPHFPQFDYSASGTA
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蛋白长度
Partial
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分子量
69.5 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
AKT1, also known as Protein Kinase B, is a critical serine/threonine kinase that plays a pivotal role in various cellular processes, including glucose metabolism, apoptosis, cell proliferation, and growth factor signaling. Its dysregulation has been implicated in numerous diseases, particularly cancer, as AKT1 is often found to be overactive or mutated in various tumor types, leading to enhanced survival and growth of cancer cells. Given its central role in the phosphoinositide 3-kinase (PI3K) signaling pathway, AKT1 has emerged as a promising target for therapeutic interventions. Research on recombinant AKT1 protein is vital, as it allows for the detailed study of its biochemical properties, regulatory mechanisms, and interactions with other signaling molecules. By producing AKT1 through recombinant DNA technology, scientists can investigate its functional role in vitro and in vivo, paving the way for the development of specific inhibitors and biomarkers for cancer diagnosis and treatment. Furthermore, understanding the dynamics of AKT1 phosphorylation and its downstream targets can elucidate the complex signaling networks involved in cellular responses to stress and growth signals. The study of recombinant AKT1 not only enhances our understanding of its physiological relevance but also contributes to the identification of potential drug targets, offering hope for novel therapeutic strategies in cancer treatment and other metabolic disorders.
