Analytical Data
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Gene name
CAPZA2
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简介
The CAPZA2 protein is a member of the F-actin capping protein family and binds to the barbed ends of actin filaments, preventing subunit exchange without severing the filaments. CAPZA2 Protein, Human (His-SUMO) is the recombinant human-derived CAPZA2 protein, expressed by E. coli , with N-6*His, N-SUMO labeled tag.
- Application
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Species
Human
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Source
E. coli
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Tag
N-6*His;N-SUMO
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P47755
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Expression Region
A2-A286
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AA Sequence
ADLEEQLSDEEKVRIAAKFIIHAPPGEFNEVFNDVRLLLNNDNLLREGAAHAFAQYNLDQFTPVKIEGYEDQVLITEHGDLGNGKFLDPKNRICFKFDHLRKEATDPRPCEVENAVESWRTSVETALRAYVKEHYPNGVCTVYGKKIDGQQTIIACIESHQFQAKNFWNGRWRSEWKFTITPSTTQVVGILKIQVHYYEDGNVQLVSHKDIQDSLTVSNEVQTAKEFIKIVEAAENEYQTAISENYQTMSDTTFKALRRQLPVTRTKIDWNKILSYKIGKEMQNA
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Protein Length
Full Length of Mature Protein
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Molecular Weight
48.8 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CAPZA2, a member of the CAPZ (capping protein of actin filaments) family, plays a crucial role in actin filament dynamics and cellular processes such as motility, morphology, and signal transduction. Recent studies have highlighted its importance in various biological contexts, including muscle development, immune response, and cancer progression. CAPZA2 functions by binding to the barbed end of actin filaments, thereby preventing further polymerization and stabilizing filament structure. This regulation of actin dynamics is essential for cellular functions, including stress response and cellular shape maintenance. Alterations in CAPZA2 expression and function have been linked to several diseases, making it a potential biomarker and therapeutic target. Advances in recombinant protein technology have enabled the production of tagged versions of CAPZA2 for further study. By using these recombinant proteins, researchers can investigate the interaction of CAPZA2 with actin and other associated proteins, providing insights into its role in the cytoskeleton and its contribution to pathophysiological conditions. Understanding the molecular mechanisms underlying CAPZA2's function may pave the way for novel therapeutic approaches to target diseases associated with aberrant actin dynamics.











