Analytical Data
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Gene name
NEK2
- Application
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Alternative Names
HsPK 21; HsPK21; NEK2A; NLK1; NimA-like protein kinase 1; Serine/threonine-protein kinase Nek2
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Species
Human
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Source
E. coli
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Tag
N-His
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
P51955
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Expression Region
Lys125~Asn393
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Molecular Weight
38kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
NEK2 (NIMA-related kinase 2) is a serine/threonine kinase that plays a critical role in cell cycle regulation, particularly during mitosis. It is part of the NEK family of kinases, which are known to be essential for maintaining chromosome stability and proper cell division. Overexpression and dysregulation of NEK2 have been implicated in various cancers, making it a potential target for therapeutic intervention. Research has shown that NEK2 is involved in multiple cellular processes, including centrosome function, cilia dynamics, and apoptosis. The study of NEK2 recombinant proteins is crucial for understanding its function at the molecular level, elucidating its interactions with other proteins, and exploring its role in tumorigenesis. By generating NEK2 recombinant proteins in vitro, researchers can delve into enzymatic activity assays, binding studies, and structural characterization, which are essential for identifying potential inhibitors or modulators of NEK2 activity. This research has the potential to contribute to the development of novel cancer treatments and improve our understanding of cancer biology, thereby addressing the pressing need for effective therapeutic strategies against malignancies associated with NEK2 dysregulation.
