Analytical Data
-
Gene name
ALK-1
-
简介
ALK-1, also known as ACVRL1, is a type I receptor for TGF-β superfamily with 2 ligands, BMP9 and BMP10. ALK-1 is predominantly expressed in endothelial cells and plays a critical role in regulating developmental and pathological angiogenesis[1][2]. ALK-1 Protein, Cynomolgus (HEK293, His) is produced in HEK293 cells with a C-Terminal His-tag. It consists of 118 amino acids (D22-Q118).
- Application
-
Biological Activity
Measured by its ability to inhibit BMP-9-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is 3.992 ng/mL in the presence of 2 ng/mL of rhBMP-9, corresponding to a specific activity is 2.505×105 U/mg. Measured by its ability to inhibit BMP-9-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is 3.992 ng/mL in the presence of 2 ng/mL of rhBMP-9, corresponding to a specific activity is 2.505×105 U/mg.
-
Alternative Names
Serine/threonine-protein kinase receptor R3; SKR3; ALK-1; TSR-I; ACVRL1
-
Species
Cynomolgus
-
Source
HEK293
-
Tag
C-His
-
Purity
Greater than 95% as determined by SDS-PAGE.
-
Uniprot
XP_005570958
-
Expression Region
D22-Q118
-
Protein Length
Partial
-
Molecular Weight
21-25 kDa.
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
ALK-1 (activin receptor-like kinase 1) is a member of the transforming growth factor-beta (TGF-β) superfamily, primarily expressed in endothelial cells and involved in vascular development and homeostasis. It plays a crucial role in angiogenesis and regulates various biological processes, including cell proliferation, differentiation, and migration. Research has shown that ALK-1 is essential for maintaining vascular stability and function, with mutations or dysregulation often linked to vascular diseases, including hereditary hemorrhagic telangiectasia (HHT) and other vascular malformations. The understanding of ALK-1's signaling pathways has led to significant insights into its role in endothelial cell biology and potential therapeutic applications. For instance, ILK-1's inhibition has been explored as a promising strategy for treating diseases characterized by abnormal angiogenesis, such as cancer and diabetic retinopathy. Additionally, recombinant ALK-1 proteins are being investigated for their potential in regenerative medicine and tissue engineering, where promoting proper vascular growth is essential for successful grafting and repair. As such, ongoing research aims to elucidate the precise mechanisms of ALK-1 signaling and its interactions with other molecular pathways, which could pave the way for novel therapeutic interventions targeting endothelial dysfunction and associated diseases. Understanding ALK-1's multifaceted roles may provide opportunities for innovative treatments that leverage its regulatory properties in vascular biology.
