Analytical Data
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Gene name
JNK3
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简介
JNK3 is a critical serine/threonine protein kinase in neuronal processes, regulating proliferation, differentiation, migration, and apoptosis. Activation through MAP2K4/MKK4 and MAP2K7/MKK7 leads to phosphorylation of JNK3, which in turn phosphorylates AP-1 components such as JUN and ATF2, thereby regulating AP-1 transcriptional activity. JNK3 Protein, Human (His) is the recombinant human-derived JNK3 protein, expressed by E. coli , with N-6*His labeled tag.
- Application
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Alternative Names
MAPK10; Mitogen-activated protein kinase 10; MAP kinase 10; MAPK 10; MAP kinase p49 3F12; Stress-activated protein kinase 1b; SAPK1b; Stress-activated protein kinase JNK3; c-Jun N-terminal kinase 3
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Species
Human
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Source
E. coli
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Tag
N-6*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P53779
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Expression Region
M1-S423
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Protein Length
Partial
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
JNK3 (c-Jun N-terminal kinase 3) is a member of the mitogen-activated protein kinase (MAPK) family, primarily involved in regulating cellular responses to stress, inflammation, and apoptosis. Research into JNK3 has gained momentum due to its significant role in various neurodegenerative diseases, such as Alzheimer's, Parkinson's, and Huntington's disease, where it has been implicated in neuronal cell death and dysfunction. JNK3 is predominantly expressed in the brain, making it a critical target for understanding neuroprotection and neuronal signaling. The study of recombinant JNK3 proteins has allowed researchers to elucidate its biochemical properties, signaling pathways, and interactions with various substrates and inhibitors. By generating recombinant JNK3, scientists aim to explore its precise functions in cellular contexts, investigate how its dysregulation contributes to neurological disorders, and identify potential therapeutic targets for drug development. Given the need for effective treatments for these debilitating conditions, ongoing research into the characterization and functionality of recombinant JNK3 proteins holds promise for advancing our understanding of neurobiology and improving therapeutic strategies.
