Analytical Data
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Gene name
IRAK1
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简介
IRAK1 is a serine/threonine protein kinase that initiates innate immune responses against pathogens through Toll-like receptor (TLR) and IL-1R signaling. After TLR activation, IRAK1 is recruited by MYD88 and undergoes IRAK4 phosphorylation, autophosphorylation, and kinase activation. IRAK1 Protein, Human (sf9, GST) is the recombinant human-derived IRAK1 protein, expressed by sf9 insect cells , with N-GST labeled tag.
- Application
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Alternative Names
IRAK1; Interleukin-1 receptor-associated kinase 1; IRAK-1
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Species
Human
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Source
Baculovirus
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Tag
N-GST
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P51617
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Expression Region
R194-S712
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Protein Length
Partial
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
IRAK1 (Interleukin-1 receptor-associated kinase 1) is a crucial component of the innate immune response, primarily involved in the signaling pathways activated by pro-inflammatory cytokines, particularly interleukin-1 (IL-1) and tumor necrosis factor-alpha (TNF-α). Dysregulation of IRAK1 has been implicated in various autoimmune diseases, chronic inflammatory conditions, and even cancer, making it a significant target for therapeutic intervention. The study of recombinant IRAK1 protein has gained attention because it facilitates a deeper understanding of its biological functions and interaction mechanisms within signaling cascades. Researchers focus on the recombinant expression and purification of IRAK1 to analyze its kinase activity, post-translational modifications, and interaction with other signaling molecules. Such studies can elucidate the role of IRAK1 in disease pathogenesis and aid in the development of IRAK1 inhibitors as potential therapeutic agents. Overall, the exploration of IRAK1 through recombinant protein research is essential for advancing our knowledge of immune regulation and improving treatment strategies for inflammatory diseases.
