Analytical Data
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Gene name
Nidogen-1
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简介
Nidogen-1 protein is a sulfated glycoprotein that is widely distributed in basement membranes and binds tightly to laminin, type IV collagen, and perlecan, suggesting multiple interactions between cells and the extracellular matrix .Nidogen-1 binds to partners such as FBLN1, LGALS3BP and PLXDC1 and plays a crucial role in the complex molecular network of the extracellular matrix.Nidogen-1 Protein, Mouse (P.pastoris, His) is the recombinant mouse-derived Nidogen-1 protein, expressed by P.pastoris , with N-His labeled tag.
- Application
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Alternative Names
Nid1; Ent; Nidogen-1; NID-1; Entactin
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Species
Mouse
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Source
P. pastoris
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P10493
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Expression Region
S428-A665
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AA Sequence
SPQRVNGKVKGRIFVGSSQVPVVFENTDLHSYVVMNHGRSYTAISTIPETVGYSLLPLAPIGGIIGWMFAVEQDGFKNGFSITGGEFTRQAEVTFLGHPGKLVLKQQFSGIDEHGHLTISTELEGRVPQIPYGASVHIEPYTELYHYSSSVITSSSTREYTVMEPDQDGAAPSHTHIYQWRQTITFQECAHDDARPALPSTQQLSVDSVFVLYNKEERILRYALSNSIGPVRDGSPDA
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Protein Length
Partial
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Molecular Weight
28.3 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Nidogen-1, also known as entactin, is a crucial extracellular matrix (ECM) glycoprotein that plays a pivotal role in maintaining the structural integrity of various tissues, including basement membranes. It serves as a linker between laminins and collagen IV, facilitating essential cellular functions such as adhesion, migration, and differentiation. Research on Nidogen-1 recombinant proteins has gained attention due to their potential therapeutic applications, particularly in tissue engineering and regenerative medicine. By producing and characterizing recombinant Nidogen-1, scientists aim to uncover its precise biological functions and interactions within the ECM. Furthermore, understanding its role in pathological conditions, such as cancer metastasis and fibrosis, can provide insights into novel treatment strategies. The development of recombinant forms of Nidogen-1 not only allows for detailed mechanistic studies but also paves the way for innovative biomaterials that mimic the natural ECM, potentially improving the outcomes of cell-based therapies and implantable devices. Overall, the investigation of Nidogen-1 and its recombinant forms is poised to advance our knowledge of ECM biology and contribute to the development of next-generation therapeutic approaches.
