Analytical Data
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Gene name
PI4KB
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简介
The PI4KB protein is a key enzyme that initiates the production of 1,4,5-trisphosphate inositol (PIP) by phosphorylating phosphatidylinositol (PI). In addition to myo-inositol signaling, it is involved in mitotic Golgi disassembly and Golgi-to-plasma membrane trafficking, which is critical for cell membrane dynamics. PI4KB Protein, Human (sf9, His, GST) is the recombinant human-derived PI4KB protein, expressed by sf9 insect cells , with N-8*His, N-GST labeled tag.
- Application
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Alternative Names
PI4KB; Phosphatidylinositol 4-kinase beta; PI4K-beta; PI4Kbeta; PtdIns 4-kinase beta; NPIK; PI4K92; PI4KIII
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Species
Human
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Source
Baculovirus
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Tag
N-8*His;N-GST
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9UBF8
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Expression Region
G2-M816
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Protein Length
Full Length of Mature Protein
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PI4KB (phosphatidylinositol 4-kinase beta) is a crucial enzyme involved in the phosphoinositide signaling pathway, specifically in the phosphorylation of phosphatidylinositol to generate phosphatidylinositol 4-phosphate (PI4P), a critical lipid involved in various cellular functions including membrane trafficking, cytoskeletal dynamics, and cellular signaling. Given its central role in these pathways, PI4KB has garnered attention in the context of various diseases, including cancer, neurodegenerative disorders, and viral infections, where dysregulation of phosphoinositide metabolism is often implicated. Researchers have focused on the structural and functional characterization of PI4KB to better understand its enzymatic mechanisms and potential as a therapeutic target. The investigation into PI4KB’s interactions, regulatory mechanisms, and its role in disease progression is vital, as it could unveil new avenues for drug development and therapeutic intervention. Thus, the study of PI4KB not only enhances our understanding of fundamental cellular processes but also lays the groundwork for innovative approaches in treating diseases linked to phosphoinositide signaling aberrations.
