Analytical Data
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Gene name
NRG1-beta 1
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简介
NRG1 is a direct ligand of the ERBB3 and ERBB4 tyrosine kinase receptors. NRG1 can induce the phosphorylation and activation of MAPK3/ERK1, MAPK1/ERK2 and AKT1. NRG1 can induce cell growth, differentiation and proliferation. NRG1-beta 1 Protein, Human (246a.a, His) is the recombinant human-derived NRG1-beta 1 protein, expressed by E. coli , with N-6*His labeled tag.
- Application
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Biological Activity
Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. The ED50 for this effect is 33.21 ng/mL, corresponding to a specific activity is 3.01×104 units/mg Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. The ED50 for this effect is 33.21 ng/mL, corresponding to a specific activity is 3.01×104 units/mg.
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Alternative Names
Pro-neuregulin-1; Neuregulin-1 beta 1; NRG1-beta 1; HRG1-beta 1 ECD; NRG1; GGF; HGL; HRGA; NDF; SMDF;
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Species
Human
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Source
E. coli
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Tag
N-6*His
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Purity
Greater than 90% as determined by reducing SDS-PAGE.
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Uniprot
AAA58639.1
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Expression Region
M1-K246
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AA Sequence
MSERKEGRGKGKGKKKERGSGKKPESAAGSQSPALPPQLKEMKSQESAAGSKLVLRCETSSEYSSLRFKWFKNGNELNRKNKPQNIKIQKKPGKSELRINKASLADSGEYMCKVISKLGNDSASANITIVESNEIITGMPASTEGAYVSSESPIRISVSTEGANTSSSTSTSTTGTSHLVKCAEKEKTFCVNGGECFMVKDLSNPSRYLCKCPNEFTGDRCQNYVMASFYKHLGIEFMEAEELYQK
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Protein Length
Partial
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Molecular Weight
35 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
NRG1-beta 1, or Neuregulin 1 beta 1, is a vital member of the neuregulin family of proteins, which play crucial roles in various physiological processes, including neuronal development, cardiac function, and immune response. Research into NRG1-beta 1 has gained significant momentum due to its involvement in crucial signaling pathways that influence cell growth, differentiation, and survival. Dysregulation of NRG1-beta 1 has been implicated in several neurodevelopmental disorders, such as schizophrenia, and cardiac conditions, highlighting its therapeutic potential. Additionally, NRG1-beta 1 has shown promise in promoting neuroprotection and repair following injury, making it a compelling target for regenerative medicine. The recombinant expression of NRG1-beta 1 enables detailed studies into its biological functions and facilitates the exploration of its applications in drug development and biotechnology. Understanding the mechanisms of NRG1-beta 1 signaling could provide insights into its therapeutic implications, paving the way for novel treatment strategies targeting a variety of diseases linked to its altered expression. As research progresses, the elucidation of the structural and functional properties of NRG1-beta 1 will enhance our knowledge and allow for the design of targeted therapies, contributing to advancements in the fields of neurology, cardiology, and beyond.
