Analytical Data
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基因名
Neurofilament light polypeptide/NEFL
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简介
Neurofilament light chain polypeptide (NEFL), like NEFM and NEFH, is an important component of neurofilaments and maintains neuronal caliber. NEFL interacts cooperatively with PRPH and INA to facilitate the assembly of neuronal filamentous networks. Neurofilament light polypeptide/NEFL, Human (His) is the recombinant human-derived Neurofilament light polypeptide/NEFL, Human (His) protein, expressed by E. coli, with C-His labeled tag.
- Application
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别名
CMT1F; CMT2E; NEFL; NF68
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种属
Human
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表达系统
E. coli
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标签
C-His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P07196
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表达区间
S2-D543
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分子量
65-80 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Neurofilament light polypeptide (NEFL) is a crucial component of the neuronal cytoskeleton, playing a significant role in maintaining the structural integrity and function of neurons. Research into NEFL has gained momentum due to its association with various neurodegenerative disorders, including amyotrophic lateral sclerosis (ALS) and Alzheimer's disease. The importance of NEFL as a potential biomarker for neuronal injury and disease progression has drawn attention, prompting studies on its expression levels in the cerebrospinal fluid and serum as indicators of neurodegeneration. Moreover, recombinant NEFL proteins are increasingly being utilized in experimental models to better understand their functional roles in neurobiology and to explore therapeutic avenues. Investigating NEFL provides insights into the pathophysiology of neuronal diseases and may facilitate the development of diagnostic tools and treatment strategies aimed at mitigating neurodegenerative processes. The production and characterization of recombinant NEFL proteins enable researchers to delve into the molecular mechanisms underlying neurofilament-related pathologies and enhance our understanding of neuronal dynamics.
