Analytical Data
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Gene name
NCL
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简介
Nucleolar protein (NCL) is the major nucleolar protein in actively growing eukaryotic cells and is associated with intranucleolar chromatin and preribosomal granules. It induces chromatin decondensation by binding to histone H1 and plays a role in pre-rRNA transcription, ribosome assembly, and potential transcription elongation. NCL Protein, Human (HEK293, mFc) is the recombinant human-derived NCL protein, expressed by HEK293, with C-mFc labeled tag.
- Application
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Alternative Names
C23; FLJ45706; MS1116 ; NCL; Nucl; Nucleolin; Protein C23
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Species
Human
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Source
HEK293
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Tag
C-mFc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P19338
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Expression Region
V2-E710
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Molecular Weight
130-150 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
NCL, or nucleolin, is a highly multifunctional protein predominantly found in the nucleolus of eukaryotic cells, playing critical roles in ribosome biogenesis, pre-mRNA processing, and cellular stress responses. Research into recombinant NCL has gained significant traction due to its potential implications in cancer and viral infections, as NCL overexpression is commonly associated with tumorigenesis and cell proliferation. Understanding the structural and functional dynamics of NCL is essential for developing targeted therapies. Recombinant protein techniques enable the production of NCL in vitro, providing a valuable tool for studying its interactions with nucleic acids and other cellular proteins. Investigating NCL's conformational variations and post-translational modifications could further elucidate its role in pathological conditions. This research also paves the way for using NCL as a biomarker for cancer diagnosis and prognosis, as well as a potential therapeutic target. With advancements in biotechnology, characterizing recombinant NCL presents opportunities to design novel inhibitors that disrupt its interactions, thus hindering cancer cell growth and viral replication. Overall, the exploration of NCL through recombinant protein technology not only enhances our fundamental understanding of nuclear processes but also promises to contribute to innovative strategies in disease management.
