Analytical Data
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Gene name
Lgr5/GPR49
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简介
The Lgr5/GPR49 protein is a receptor for R-spondins that enhances the canonical Wnt signaling pathway and marks stem cells in the intestinal epithelium and hair follicles. Lgr5/GPR49 binds R-spondins (RSPO1-4), binds to phosphorylated LRP6 and Frizzled receptors, initiates Wnt signaling and amplifies target gene expression. Lgr5/GPR49 Protein, Human (540a.a, HEK293, Fc) is the recombinant human-derived Lgr5/GPR49 protein, expressed by HEK293, with C-hFc labeled tag.
- Application
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Alternative Names
LGR5; GPR49; GPR67; HG38; G-protein coupled receptor 49; G-protein coupled receptor 67; G-protein coupled receptor HG38
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Species
Human
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Source
HEK293
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Tag
C-hFc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O75473-1
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Expression Region
G22-R561
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Molecular Weight
95-100 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Lgr5, also known as GPR49, is a member of the leucin-rich repeat-containing G protein-coupled receptor family and has garnered significant attention in the field of stem cell research and regenerative medicine. Initially identified as a marker for intestinal stem cells, Lgr5 plays a crucial role in maintaining tissue homeostasis and promoting cellular proliferation in various organs, including the intestine, skin, and hair follicles. Its expression is closely associated with Wnt signaling pathways, which are critical for stem cell activity and differentiation. Recent studies suggest that Lgr5/GPR49 may also have implications in cancer biology, particularly in the context of tumor stem cells and their role in tumorigenesis and metastasis. The characterization of Lgr5/GPR49 recombinant proteins enables researchers to explore its functional properties, interactions with ligands, and potential as a therapeutic target. Understanding the signaling mechanisms and biological functions of Lgr5 could provide new insights into stem cell niche dynamics and inform strategies for tissue regeneration and cancer treatment. As research progresses, the modulation of Lgr5/GPR49 pathways offers promising avenues for enhancing regenerative capabilities and developing novel interventions for various diseases.
