Analytical Data
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Gene name
Betacellulin/BTC
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简介
Betacellulin/BTC Protein, a growth factor with affinity for EGFR, ERBB4, and other EGF receptor family members, exerts potent mitogenic effects on retinal pigment epithelial cells and vascular smooth muscle cells as a monomer. Its molecular activity involves interactions with EGFR and ERBB4, contributing to cellular signaling and regulation. Betacellulin/BTC Protein, Mouse (HEK293, His-hFc) is the recombinant mouse-derived Betacellulin/BTC protein, expressed by HEK293 , with C-hFc, C-His labeled tag.
- Application
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Alternative Names
Btc; Betacellulin; Betacellulin epidermal growth factor family member
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Species
Mouse
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Source
HEK293
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Tag
C-hFc;C-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q05928
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Expression Region
D32-Q118
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Protein Length
Extracellular Domain
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Molecular Weight
50-55 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Betacellulin (BTC) is a member of the epidermal growth factor (EGF) family, known for its crucial role in regulating pancreatic beta-cell functions and promoting insulin secretion, making it a significant target in diabetes research. It was initially discovered in pancreatic tissues and has been found to have mitogenic effects on beta-cells, contributing to their proliferation and differentiation. Researchers are particularly interested in BTC due to its potential applications in the treatment of type 2 diabetes, where beta-cell dysfunction is a prominent feature. The ability of BTC to enhance beta-cell survival and function suggests that recombinant BTC proteins could be developed as therapeutic agents. Several studies have explored the molecular mechanisms underlying BTC's action, highlighting its interaction with EGF receptors and downstream signaling pathways that govern insulin biosynthesis and release. Ongoing research is focused on optimizing the production and delivery of recombinant BTC protein, aiming to evaluate its efficacy in preclinical models of diabetes. By enhancing our understanding of BTC’s biological functions and therapeutic potential, these studies aim to pave the way for novel treatments that could restore beta-cell function and improve metabolic control in diabetic patients.
