Analytical Data
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Gene name
KMT2D
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简介
KMT2D Protein, a histone methyltransferase, methylates histone H3 'Lys-4' (H3K4), predominantly establishing H3K4me1 marks at active chromatin sites. Integral to chromatin remodeling, it functions as a coactivator for the estrogen receptor, recruited by ESR1, activating transcription. KMT2D's role in depositing specific histone marks at genomic locations underscores its crucial involvement in modulating chromatin structure and gene expression. KMT2D Protein, Human is the recombinant human-derived KMT2D protein, expressed by E. coli , with tag free.
- Application
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Alternative Names
KMT2D; Histone-lysine N-methyltransferase 2D; Lysine N-methyltransferase 2D; ALL1-related protein; Myeloid/lymphoid or mixed-lineage leukemia protein 2
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Species
Human
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Source
E. coli
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Tag
Tag Free
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O14686
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Expression Region
H5382-M5536
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Protein Length
Partial
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
KMT2D (also known as MLL2) is a key epigenetic regulator involved in histone methylation, specifically the trimethylation of histone H3 at lysine 4 (H3K4me3), which is crucial for gene activation and transcriptional regulation. Mutations or alterations in the KMT2D gene have been implicated in various developmental disorders and diseases, including Kabuki syndrome, which is characterized by distinctive facial features, growth delays, and intellectual disability. Research into KMT2D and its associated protein has gained momentum due to its essential role in the regulation of gene expression during embryonic development and its potential implications in cancer biology. Recombinant KMT2D proteins are utilized in studies to understand the protein's structure-function relationship, its interaction with other epigenetic modifiers, and its overall impact on chromatin dynamics. By producing and analyzing these recombinant proteins, researchers aim to elucidate the molecular mechanisms underlying KMT2D function and its contributions to both normal development and disease pathology. This investigation not only enhances our understanding of epigenetic regulation but also opens avenues for potential therapeutic interventions targeting KMT2D-related conditions.
