Analytical Data
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Gene name
EphA8
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简介
The EPHA8 protein is a receptor tyrosine kinase that promiscuously binds to the GPI-anchored ephrin-A ligand to initiate bidirectional signaling. It participates in forward and reverse signaling pathways and is phosphorylated by ligands such as EFNA2, EFNA3, and EFNA5. EphA8 Protein, Human (HEK293, His) is the recombinant human-derived EphA8 protein, expressed by HEK293 , with C-6*His labeled tag.
- Application
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Alternative Names
rHuEphrin type-A receptor 8/EphA8, His; EEK; EK3; HEK3; EPH- and ELK-related kinase; EPH- and ELK-related tyrosine kinase; EPH receptor A8; EphA8; EPH-like kinase 3; ephrin type-A receptor 8; Hek3
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Species
Human
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Source
HEK293
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Tag
C-6*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P29322-1
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Expression Region
E31-T542
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AA Sequence
EVNLLDTSTIHGDWGWLTYPAHGWDSINEVDESFQPIHTYQVCNVMSPNQNNWLRTSWVPRDGARRVYAEIKFTLRDCNSMPGVLGTCKETFNLYYLESDRDLGASTQESQFLKIDTIAADESFTGADLGVRRLKLNTEVRSVGPLSKRGFYLAFQDIGACLAILSLRIYYKKCPAMVRNLAAFSEAVTGADSSSLVEVRGQCVRHSEERDTPKMYCSAEGEWLVPIGKCVCSAGYEERRDACVACELGFYKSAPGDQLCARCPPHSHSAAPAAQACHCDLSYYRAALDPPSSACTRPPSAPVNLISSVNGTSVTLEWAPPLDPGGRSDITYNAVCRRCPWALSRCEACGSGTRFVPQQTSLVQASLLVANLLAHMNYSFWIEAVNGVSDLSPEPRRAAVVNITTNQAAPSQVVVIRQERAGQTSVSLLWQEPEQPNGIILEYEIKYYEKDKEMQSYSTLKAVTTRATVSGLKPGTRYVFQVRARTSAGCGRFSQAMEVETGKPRPRYDTRT
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Protein Length
Extracellular Domain
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Molecular Weight
65-80 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
EphA8 is a member of the Eph receptor tyrosine kinase family, which plays crucial roles in various biological processes, including cell adhesion, migration, and tissue development. The EphA8 receptor is particularly notable for its involvement in neural development and angiogenesis, making it a significant target for research in neurobiology and cancer. Its expression patterns and functional implications in different tissues have drawn attention, as dysregulation of EphA8 signaling pathways has been linked to various diseases, including tumors and neurological disorders. The study of recombinant EphA8 protein holds promise for uncovering its structural and functional properties, facilitating the understanding of its interactions with ligands such as ephrin-A. Moreover, the generation of recombinant EphA8 provides a valuable tool for elucidating the mechanisms behind EphA8-mediated signaling and its potential as a therapeutic target. By employing techniques like protein purification and characterization, researchers aim to explore the biochemical aspects of EphA8, suggesting its role in cell signaling pathways. Overall, the investigation of EphA8 recombinant protein represents a significant step towards developing novel strategies for targeted therapies in diseases where EphA8 is implicated.
