Analytical Data
-
Gene name
MAN1B1
-
简介
MAN1B1, integral to glycoprotein quality control, trims a single alpha-1,2-linked mannose from Man(9)GlcNAc(2), producing Man(8)GlcNAc(2). In the ERQC, elevated enzyme concentrations allow further trimming to Man(5-6)GlcNAc(2). This enzymatic function is crucial for glycoprotein processing and quality control in the endoplasmic reticulum, ensuring proper folding and maturation. MAN1B1 Protein, Human (HEK293, His) is the recombinant human-derived MAN1B1 protein, expressed by HEK293 , with C-6*His labeled tag.
- Application
-
Species
Human
-
Source
HEK293
-
Tag
C-6*His
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
Q9UKM7
-
Expression Region
D106-A699
-
AA Sequence
DHWKALAFRLEEEQKMRPEIAGLKPANPPVLPAPQKADTDPENLPEISSQKTQRHIQRGPPHLQIRPPSQDLKDGTQEEATKRQEAPVDPRPEGDPQRTVISWRGAVIEPEQGTELPSRRAEVPTKPPLPPARTQGTPVHLNYRQKGVIDVFLHAWKGYRKFAWGHDELKPVSRSFSEWFGLGLTLIDALDTMWILGLRKEFEEARKWVSKKLHFEKDVDVNLFESTIRILGGLLSAYHLSGDSLFLRKAEDFGNRLMPAFRTPSKIPYSDVNIGTGVAHPPRWTSDSTVAEVTSIQLEFRELSRLTGDKKFQEAVEKVTQHIHGLSGKKDGLVPMFINTHSGLFTHLGVFTLGARADSYYEYLLKQWIQGGKQETQLLEDYVEAIEGVRTHLLRHSEPSKLTFVGELAHGRFSAKMDHLVCFLPGTLALGVYHGLPASHMELAQELMETCYQMNRQMETGLSPEIVHFNLYPQPGRRDVEVKPADRHNLLRPETVESLFYLYRVTGDRKYQDWGWEILQSFSRFTRVPSGGYSSINNVQDPQKPEPRDKMESFFLGETLKYLFLLFSDDPNLLSLDAYVFNTEAHPLPIWTPA
-
Protein Length
Lumenal Domain
-
Molecular Weight
58-80 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
MAN1B1, or Mannosidase 1B member 1, is a crucial enzyme involved in the maturation of glycoproteins through the removal of mannose residues during N-glycan processing in the endoplasmic reticulum. Its role in protein glycosylation is vital, as this post-translational modification significantly influences protein folding, stability, and cell signaling. Dysfunctions in MAN1B1 have been linked to various diseases, including congenital disorders of glycosylation and certain cancers, highlighting its importance in human health. Recent studies have focused on the recombinant production of MAN1B1 to facilitate detailed biochemical characterization and to explore its potential as a therapeutic target. The ability to produce this enzyme in a recombinant form allows researchers to investigate its mechanisms, develop inhibitors, and assess its role in disease progression. Furthermore, understanding MAN1B1's structure-function relationship will enable advancements in the design of biopharmaceuticals with improved efficacy and safety profiles. Overall, the study of MAN1B1 and its recombinant protein presents significant implications for both basic research and clinical applications in treating glycosylation-related diseases.
