Analytical Data
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Gene name
HYOU1
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简介
The HYOU1 protein plays a crucial role in coordinating cytoprotective mechanisms in response to hypoxia, indicating its importance in cellular stress responses. It involves a potential molecular chaperone function and is actively involved in the protein folding process. HYOU1 Protein, Human (HEK293, His) is the recombinant human-derived HYOU1 protein, expressed by HEK293 , with C-10*His labeled tag.
- Application
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Alternative Names
Hypoxia up-regulated protein 1; 150 kDa oxygen-regulated protein; ORP-150; 170 kDa glucose-regulated protein; GRP-170; HYOU1; ORP150
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Species
Human
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Source
HEK293
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Tag
C-10*His
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
Q9Y4L1-1
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Expression Region
M695-L999
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AA Sequence
MVEEIGVELVVLDLPDLPEDKLAQSVQKLQDLTLRDLEKQEREKAANSLEAFIFETQDKLYQPEYQEVSTEEQREEISGKLSAASTWLEDEGVGATTVMLKEKLAELRKLCQGLFFRVEERKKWPERLSALDNLLNHSSMFLKGARLIPEMDQIFTEVEMTTLEKVINETWAWKNATLAEQAKLPATEKPVLLSKDIEAKMMALDREVQYLLNKAKFTKPRPRPKDKNGTRAEPPLNASASDQGEKVIPPAGQTEDAEPISEPEKVETGSEPGDTEPLELGGPGAEPEQKEQSTGQKRPLKNDEL
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Protein Length
Partial
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Molecular Weight
53-75 kDa,.
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
HYOU1, also known as hypoxia up-regulated 1, is a protein that has garnered significant attention in the field of molecular biology and medicine due to its role in cellular response to stress, particularly in hypoxic conditions. This protein is associated with the endoplasmic reticulum (ER) and plays a crucial role in protein folding and cellular homeostasis. Research has demonstrated that HYOU1 is upregulated in various pathological conditions, including cancer, where it contributes to tumor growth and survival by enhancing the adaptability of cancer cells to hypoxic environments. Moreover, HYOU1 is thought to be involved in the regulation of the unfolded protein response (UPR), a protective mechanism activated under stress conditions that aims to restore normal cell function. This makes HYOU1 a potential target for therapeutic strategies aimed at modulating the UPR in diseases characterized by hypoxia or enhanced ER stress. The study of HYOU1 as a recombinant protein allows for a deeper understanding of its structure, function, and interactions within the cell, potentially leading to novel approaches in cancer treatment and other diseases where hypoxic conditions prevail. Given its importance in both normal physiology and disease pathology, ongoing research into the functional mechanisms of HYOU1 and its potential applications in clinical settings is vital for the advancement of therapeutic interventions.
