Analytical Data
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Gene name
UBE2O
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简介
UBE2O protein acts as a hybrid E2/E3 ubiquitin protein ligase capable of monoubiquitinating target proteins. It negatively regulates TRAF6-mediated NF-kappa-B activation independently of E2 activity. UBE2O Protein, Human (sf9, His, Strep) is the recombinant human-derived UBE2O protein, expressed by sf9 insect cells , with N-Strep, N-8*His labeled tag.
- Application
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Species
Human
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Source
Baculovirus
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Tag
N-StrepⅡ;N-8*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9C0C9
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Expression Region
A2-K1292
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Protein Length
Partial
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
UBE2O, a member of the E2 ubiquitin-conjugating enzyme family, plays a critical role in the ubiquitin-proteasome system, which regulates protein degradation and various cellular processes. UBE2O specifically is known for its involvement in the monoubiquitination of substrates, contributing to key biological functions such as cell cycle regulation, DNA repair, and stress responses. Recent studies have highlighted UBE2O's potential implications in cancer biology, particularly in tumor progression and resistance to therapy, suggesting that its dysregulation may contribute to malignancies. Additionally, UBE2O has been implicated in the pathogenesis of neurodegenerative diseases, where protein aggregation is a central feature. The recombinant expression of UBE2O allows researchers to investigate its enzymatic activity, substrate specificity, and interaction with other proteins in greater detail. Understanding the function of UBE2O at the molecular level could provide insights into its role in disease mechanisms and pave the way for the development of targeted therapeutic strategies. Overall, the study of UBE2O and its recombinant protein forms holds promise for uncovering novel aspects of cellular regulation and potential clinical applications in treating diseases characterized by proteostasis disruption.
