Analytical Data
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基因名
CDK3-CCNE1 Heterodimer
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简介
CDK3 protein is a serine/threonine protein kinase that plays a key role in regulating the eukaryotic cell cycle, especially affecting the G0-G1 and G1-S transitions. It interacts with CCNC/cyclin-C during interphase and phosphorylates substrates such as histone H1, ATF1, RB1, and CABLES1. CDK3-CCNE1 Heterodimer Protein, Human (sf9) is a recombinant protein dimer complex containing human-derived CDK3-CCNE1 Heterodimer protein, expressed by sf9 insect cells , with tag free.
- Application
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别名
CDK3; CCNE1; Cyclin-dependent kinase 3; Cell division protein kinase 3; G1/S-specific cyclin-E1
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种属
Human
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表达系统
Baculovirus
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标签
Tag Free
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q00526 (M1-H305)&P24864
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表达区间
Q00526 (M1-H305)&P24864 (M1-E410)
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CDK3-CCNE1 heterodimer is a crucial protein complex involved in the regulation of the cell cycle, particularly during the transition from the G1 phase to the S phase. Cyclin E1 (CCNE1) is a regulatory subunit that activates Cyclin-dependent kinase 3 (CDK3), facilitating the phosphorylation of key substrates that drive cellular progression. Dysregulation of this complex has been implicated in various cancers, where abnormal cell cycle progression can lead to unchecked cellular proliferation. Understanding the structure and function of the CDK3-CCNE1 heterodimer is vital for elucidating the molecular mechanisms underlying cell cycle control and tumorigenesis. Researchers are increasingly focused on characterizing this protein complex, including its interaction dynamics, the structural basis for substrate recognition, and its role in oncogenic signaling pathways. By employing advanced techniques such as X-ray crystallography and cryo-electron microscopy, studies aim to unveil the intricate details of CDK3-CCNE1 function. This knowledge may pave the way for developing targeted therapeutic strategies to disrupt aberrant cell cycle regulation in cancer, offering potential for novel interventions in cancer treatment.
