Analytical Data
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Gene name
MAP4K2
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简介
MAP4K2 is a serine/threonine protein kinase and an important component of the MAP kinase signal transduction pathway. It acts as a MAPK kinase kinase (MAP4K), activating the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) pathway and, to a lesser extent, the p38 MAPKs pathway. MAP4K2 Protein, Human (sf9, His-GST) is the recombinant human-derived MAP4K2 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.
- Application
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Alternative Names
Mitogen-activated protein kinase kinase kinase kinase 2; GC kinase; MEKKK 2; GCK; RAB8IP
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Species
Human
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Source
Baculovirus
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Tag
N-His;N-GST
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q12851-2
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Expression Region
M1-Y812
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Protein Length
Full Length of Isoform-2
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Molecular Weight
116 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
MAP4K2 (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 2) is a member of the MAP4K family, which plays a crucial role in the regulation of cellular signaling pathways, particularly those involved in stress responses, cell proliferation, and apoptosis. This kinase has garnered substantial interest due to its potential implications in various diseases, including cancer and neurodegenerative disorders. Research indicates that MAP4K2 is involved in the activation of MAPK pathways, which are pivotal for cellular responses to external stimuli. Recent studies have highlighted that alterations in MAP4K2 expression and activity can contribute to the development and progression of tumors, suggesting its role as a potential oncogene. Furthermore, its involvement in neuronal signaling raises questions about its function in brain health and disease. Recombining MAP4K2 protein in various biological systems can provide insights into its intricate role in cell signaling and disease mechanisms. Investigating its biochemical properties, interaction partners, and regulatory mechanisms could lead to the identification of novel therapeutic targets, particularly in malignancies where MAP4K2 is dysregulated. Thus, studying MAP4K2 through recombinant protein techniques holds promise for advancing our understanding of MAPK signaling and its implications in health and disease, potentially paving the way for innovative treatment strategies.
