Analytical Data
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基因名
Apolipoprotein E/APOE4
- Application
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别名
Apolipoprotein E; Apo-E; APOE; apolipo E; APOE4
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种属
Human
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表达系统
HEK293
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标签
N-8*His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
AAB59397.1
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表达区间
K19-H317
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蛋白长度
Full Length of Mature Protein
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分子量
30-40 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Apolipoprotein E (ApoE) is a crucial protein involved in lipid metabolism and neurodegenerative diseases, particularly Alzheimer’s disease (AD). The ApoE gene exists in three major isoforms: ApoE2, ApoE3, and ApoE4, with ApoE4 being a significant genetic risk factor for late-onset AD. Research indicates that individuals possessing the ApoE4 allele have a higher likelihood of developing AD and an earlier onset of cognitive decline compared to those with other isoforms. The pathogenic mechanisms associated with ApoE4 are not fully understood but are believed to relate to altered lipid transport, neuroinflammation, and amyloid-beta deposition in the brain. The study of recombinant ApoE4 proteins is pivotal for elucidating these mechanisms. By isolating and characterizing recombinant ApoE4, researchers can investigate its structural properties, interaction with lipids and receptors, and its impact on cellular processes. Moreover, understanding the differences between ApoE isoforms can aid in developing targeted therapies and interventions for patients at risk for AD. Exploring recombinant ApoE4 also offers insights into potential biomarker development and novel therapeutic strategies that could mitigate its deleterious effects in neurodegenerative diseases. Overall, the investigation of recombinant ApoE4 proteins contributes significantly to the understanding of ApoE's role in lipid metabolism and its implications for Alzheimer's pathology.
