Analytical Data
-
基因名
NAMPT
-
简介
NAMPT protein catalyzes the synthesis of nicotinamide mononucleotide, a key intermediate in NAD biosynthesis. NAMPT Protein, Human (sf9, His, GST) is the recombinant human-derived NAMPT protein, expressed by Sf9 insect cells, with N-His and N-GST labeled tag.
- Application
-
别名
rHuNicotinamide phosphoribosyltransferase/NAMPT, His; Pre-B cell-enhancing factor; Nicotinamide phosphoribosyltransferase; NAmPRTase; Nampt; Pre-B-cell colony-enhancing factor 1; Visfatin; NAMPT; PBEF; PBEF1
-
种属
Human
-
表达系统
Baculovirus
-
标签
N-His;N-GST
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
蛋白编号
P43490
-
表达区间
M1-H491
-
氨基酸序列
MNPAAEAEFNILLATDSYKVTHYKQYPPNTSKVYSYFECREKKTENSKLRKVKYEETVFYGLQYILNKYLKGKVVTKEKIQEAKDVYKEHFQDDVFNEKGWNYILEKYDGHLPIEIKAVPEGFVIPRGNVLFTVENTDPECYWLTNWIETILVQSWYPITVATNSREQKKILAKYLLETSGNLDGLEYKLHDFGYRGVSSQETAGIGASAHLVNFKGTDTVAGLALIKKYYGTKDPVPGYSVPAAEHSTITAWGKDHEKDAFEHIVTQFSSVPVSVVSDSYDIYNACEKIWGEDLRHLIVSRSTQAPLIIRPDSGNPLDTVLKVLEILGKKFPVTENSKGYKLLPPYLRVIQGDGVDINTLQEIVEGMKQKMWSIENIAFGSGGGLLQKLTRDLLNCSFKCSYVVTNGLGINVFKDPVADPNKRSKKGRLSLHRTPAGNFVTLEEGKGDLEEYGQDLLHTVFKNGKVTKSYSFDEIRKNAQLNIELEAAHH
-
蛋白长度
Full Length
-
分子量
75 kDa
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Nicotinamide adenine dinucleotide (NAD) metabolism plays a crucial role in various biological processes, and NAMPT (Nicotinamide Phosphoribosyltransferase) is a key enzyme in this pathway, catalyzing the conversion of nicotinamide to nicotinamide mononucleotide, a precursor of NAD. Research on NAMPT has gained significant attention due to its implications in cellular energy metabolism, aging, and various diseases, including cancer, diabetes, and neurodegenerative disorders. Dysregulation of NAMPT expression and activity has been linked to altered NAD levels, which can affect cellular functions such as DNA repair, mitochondrial function, and apoptosis. As a result, NAMPT is considered a potential therapeutic target for modulating NAD levels and improving metabolic health. Studies focusing on the purification and characterization of recombinant NAMPT proteins have facilitated a better understanding of its enzymatic mechanisms, substrate specificity, and interactions with other cellular pathways. This has opened avenues for the development of NAMPT inhibitors or activators that could be used in therapeutic contexts to address diseases associated with altered NAD metabolism.
