Analytical Data
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基因名
CROT
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简介
CROT protein is critical in the β-oxidation process, showing the highest enzymatic activity on C6 to C10 substrates. As a key player in lipid metabolism, CROT promotes the conversion of 4,8-dimethylnonanoyl-CoA to its carnitine ester, regulating fatty acid oxidation to achieve cellular energy balance. CROT Protein, Human (V474L, sf9, His) is the recombinant human-derived CROT protein, expressed by Sf9 insect cells , with C-His labeled tag and V474L mutation.
- Application
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别名
Peroxisomal carnitine O-octanoyltransferase; CROT; COT
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种属
Human
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表达系统
Baculovirus
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标签
C-His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9UKG9-1
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表达区间
M1-L612, V474L
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蛋白长度
Full Length of Isoform-1
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分子量
65 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CROT (Carnitine O-acetyltransferase) is an important enzyme involved in the metabolic pathway of fatty acid oxidation, specifically in the transport of fatty acids into the mitochondria for energy production. Recent research has highlighted its potential role in various metabolic disorders and diseases, such as obesity, diabetes, and cardiovascular conditions. The investigation into the recombinant production of CROT protein aims to facilitate a deeper understanding of its structure-function relationship and regulatory mechanisms in cellular metabolism. By producing CROT in a recombinant system, scientists can obtain large quantities of the protein for detailed biochemical analyses, including enzymatic activity assays and interaction studies with other metabolic enzymes. Furthermore, characterizing the recombinant CROT could unveil its potential as a therapeutic target or diagnostic biomarker in metabolic syndrome. The enhanced availability of this protein is also poised to accelerate the development of small-molecule drugs that can modulate its activity, presenting novel approaches to treat metabolic disorders. Ultimately, the research on recombinant CROT not only sheds light on fundamental metabolic processes but also lays the groundwork for innovative therapeutic strategies that could transform the management of metabolic diseases.
