Analytical Data
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Gene name
CROT
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简介
CROT protein is critical in the β-oxidation process, showing the highest enzymatic activity on C6 to C10 substrates. As a key player in lipid metabolism, CROT promotes the conversion of 4,8-dimethylnonanoyl-CoA to its carnitine ester, regulating fatty acid oxidation to achieve cellular energy balance. CROT Protein, Human (V474L, sf9, His) is the recombinant human-derived CROT protein, expressed by Sf9 insect cells , with C-His labeled tag and V474L mutation.
- Application
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Alternative Names
Peroxisomal carnitine O-octanoyltransferase; CROT; COT
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Species
Human
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Source
Baculovirus
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Tag
C-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9UKG9-1
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Expression Region
M1-L612, V474L
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Protein Length
Full Length of Isoform-1
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Molecular Weight
65 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CROT (Carnitine O-acetyltransferase) is an important enzyme involved in the metabolic pathway of fatty acid oxidation, specifically in the transport of fatty acids into the mitochondria for energy production. Recent research has highlighted its potential role in various metabolic disorders and diseases, such as obesity, diabetes, and cardiovascular conditions. The investigation into the recombinant production of CROT protein aims to facilitate a deeper understanding of its structure-function relationship and regulatory mechanisms in cellular metabolism. By producing CROT in a recombinant system, scientists can obtain large quantities of the protein for detailed biochemical analyses, including enzymatic activity assays and interaction studies with other metabolic enzymes. Furthermore, characterizing the recombinant CROT could unveil its potential as a therapeutic target or diagnostic biomarker in metabolic syndrome. The enhanced availability of this protein is also poised to accelerate the development of small-molecule drugs that can modulate its activity, presenting novel approaches to treat metabolic disorders. Ultimately, the research on recombinant CROT not only sheds light on fundamental metabolic processes but also lays the groundwork for innovative therapeutic strategies that could transform the management of metabolic diseases.
