Analytical Data
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Gene name
TNF-alpha/TNFSF2
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简介
TNF-alpha/TNFSF2 protein binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It induces tumor cell death, fever, cachexia, cell proliferation, and cell differentiation. It also causes insulin resistance and GKAP42 protein degradation in adipocytes. Additionally, it plays a role in angiogenesis, osteoclastogenesis, and IL12 production in dendritic cells. TNF-alpha/TNFSF2 Protein, Cynomolgus (N-His) is the recombinant cynomolgus-derived TNF-alpha/TNFSF2 protein, expressed by E. coli , with N-6*His labeled tag.
- Application
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Biological Activity
Measured in a cytotoxicity assay using L929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 this effect is 10-15 ng/mL. Measured in a cytotoxicity assay using L929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 this effect is 11.55 ng/mL, corresponding to a specific activity is 8.658×104 units/mg.
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Alternative Names
Tumor Necrosis Factor; Cachectin; TNF-Alpha; Tnf; Tnfa; Tnfsf2; ICD1
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Species
Cynomolgus
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Source
E. coli
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Tag
N-6*His
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Purity
Greater than 95% as determined by reducing SDS-PAGE.
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Uniprot
P79337
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Expression Region
V77-L233
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Protein Length
Partial
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Molecular Weight
19-25 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Tumor Necrosis Factor-alpha (TNF-alpha), also known as TNFSF2, is a pivotal pro-inflammatory cytokine involved in various physiological and pathological processes, including immune response, cell proliferation, apoptosis, and inflammation. Its dysregulation is implicated in several diseases, such as autoimmune disorders, cancer, and chronic inflammatory conditions. Understanding the biological mechanisms of TNF-alpha has drawn considerable interest within the scientific community, leading to the development of therapeutic agents targeting its signaling pathways. Recombinant TNF-alpha proteins have been produced for research purposes to study their roles in cell signaling and as potential therapeutic targets. These recombinant proteins enable researchers to investigate the functional variations of TNF-alpha, its receptor interactions, and the downstream signaling pathways it activates. Furthermore, they facilitate the assessment of pharmacological interventions aimed at modulating TNF-alpha activity. The exploration of TNF-alpha's role in disease contexts has underscored the significance of this cytokine in medical research, leading to the development of TNF inhibitors as effective treatments for inflammatory diseases. Overall, the study of TNF-alpha/TNFSF2 recombinant proteins is critical for unveiling novel insights into immune regulation and developing targeted therapies for a range of inflammatory conditions.
