Analytical Data
-
Gene name
PYHIN1
-
简介
PYHIN1 Protein, a major mediator of IFN's tumor suppressor activity in breast cancer cells, facilitates the ubiquitination and degradation of MDM2, leading to p53/TP53 stabilization. It also promotes the ubiquitination and degradation of HDAC1, enhancing maspin expression and impairing the invasive activity of cancer cells. PYHIN1 interacts with MDM2 in these regulatory processes. PYHIN1 Protein, Human (His, Strep) is the recombinant human-derived PYHIN1 protein, expressed by E. coli , with N-Strep, N-6*His labeled tag.
- Application
-
Alternative Names
PYHIN1; Pyrin and HIN domain-containing protein 1; Interferon-inducible protein X
-
Species
Human
-
Source
E. coli
-
Tag
C-Flag;N-StrepⅡ;N-6*His
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
Q6K0P9
-
Expression Region
M1-P492
-
Protein Length
Full Length
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The study of the PYHIN1 (Pyrin and HIN domain-containing protein 1) recombinant protein has gained significant attention due to its potential roles in the immune response and inflammation. PYHIN1 is a member of the HIN (hematopoietic interferon-inducible nuclear protein) family and has been implicated in various biological processes, including the regulation of type I interferon production and modulation of the innate immune response. Researchers have noted its crucial involvement in sensing cytoplasmic DNA, which can trigger immune activation and participate in the pathogenesis of autoimmune diseases. The recombinant form of PYHIN1 allows for detailed investigations into its structural properties, functional mechanisms, and interactions with other cellular components. By producing this protein in vitro, scientists can assess its activity, investigate its role in immune signaling pathways, and explore its therapeutic potential in treating inflammatory diseases. Recent studies have also highlighted PYHIN1's involvement in cancer biology, suggesting that it may play a dual role in tumor suppression and promotion depending on the cellular context. Understanding PYHIN1 through recombinant technology not only expands the knowledge of innate immunity but also opens avenues for novel therapeutic strategies targeting PYHIN1-related pathways in various diseases, making it a promising subject for future biomedical research.
