Analytical Data
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基因名
CDK7-CCNH-MNAT1
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简介
Together with its regulatory partners CCNH and MNAT1, CDK7 functions as an important serine/threonine kinase in cell cycle regulation and RNA polymerase II-mediated transcription. As the catalytic subunit of the CDK-activated kinase (CAK) complex, CDK7 plays a key role in activating CDK1/cyclin-B and CDK2/cyclin during cell cycle transitions. CDK7-CCNH-MNAT1 Protein, Human (sf9, His) is the recombinant human-derived CDK7-CCNH-MNAT1 protein, expressed by Sf9 insect cells , with N-His labeled tag. CDK7-CCNH-MNAT1 Protein, Human (sf9, His), has molecular weight of ~25 & 38 & 44 kDa, respectively.
- Application
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别名
CAK1 Protein; CDKN7 Protein; HCAK Protein; MO15 Protein; p39MO15 Protein; STK1 Protein
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种属
Human
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表达系统
Baculovirus
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标签
N-His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P50613 (A2-F346)&P51946 (Y2-L323)&P51948
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表达区间
P50613 (A2-F346)&P51946 (Y2-L323)&P51948 (D2-S309)
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分子量
25 kDa & 38 kDa & 44 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CDK7-CCNH-MNAT1 recombinant protein is an important player in the field of cell cycle regulation and transcriptional control. CDK7, a cyclin-dependent kinase, is part of the transcription factor TFIIH and plays a dual role in both cell cycle progression and the phosphorylation of the RNA polymerase II C-terminal domain, thus facilitating mRNA synthesis. CCNH, or Cyclin H, is essential for the activation of CDK7 and aids in the stabilization of the CDK7-cyclin complex, making it critical for its kinase activity. MNAT1 (also known as MNAT1 or CDK-activating kinase (CAK)) further enhances the function of CDK7 by phosphorylating it, ensuring proper activation and function during transcription initiation and cell division. Together, the CDK7-CCNH-MNAT1 complex plays a pivotal role in maintaining normal cellular functions, and aberrations in this system can lead to various pathologies, including cancer. Studying the recombinant forms of these proteins allows researchers to dissect their individual contributions and interactions in biochemical assays, potentially identifying novel therapeutic targets for diseases characterized by dysregulated cell cycle and transcriptional machinery.
