Analytical Data
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Gene name
TFIIF-associating CTD phosphatase
- Application
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Alternative Names
TFIIF-associating CTD phosphatase
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Species
Mouse
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Source
E. coli
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Tag
N- His & C- Myc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q7TSG2
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Expression Region
178-341aa
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Molecular Weight
24 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
TFIIF-associating CTD phosphatase (TFIIF-CTD phosphatase) is a vital enzyme involved in the regulation of RNA polymerase II (Pol II) transcription, specifically through its interaction with the carboxy-terminal domain (CTD) of the Pol II large subunit. The phosphorylation state of the CTD plays a crucial role in the transition between transcription initiation and elongation, influencing gene expression and cellular responses. TFIIF, a key transcription factor, associates with the CTD, and the phosphatase’s role is to dephosphorylate specific serine residues within this domain, facilitating the recycling of Pol II and the proper progression of the transcription cycle. Given its significance in transcription regulation, abnormalities in CTD phosphorylation can lead to various diseases, including cancer. Therefore, understanding the function and mechanism of TFIIF-CTD phosphatase is essential for uncovering the intricacies of gene regulation and its implications in disease. Moreover, the production of recombinant forms of this phosphatase enables detailed biochemical analysis and functional studies, paving the way for potential therapeutic strategies aimed at modulating transcriptional activity. Overall, research on TFIIF-associating CTD phosphatase not only elucidates fundamental transcriptional mechanisms but also provides insights into its roles in health and disease, highlighting its potential as a drug target for therapeutic interventions.
