Analytical Data
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基因名
HGPRT
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简介
HGPRT proteins play a key role in purine metabolism, promoting the conversion of guanine to guanosine monophosphate and hypoxanthine to inosine monophosphate. Through its enzymatic activity, HGPRT transfers the 5-phosphate ribosyl group from 5-phosphate ribose pyrophosphate to purine, making a significant contribution to the purine salvage pathway. HGPRT Protein, Human (His) is the recombinant human-derived HGPRT protein, expressed by E. coli , with N-6*His labeled tag.
- Application
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别名
rHuHypoxanthine-guanine phosphoribosyltransferase/HGPRT, His; Hypoxanthine-Guanine Phosphoribosyltransferase; HGPRT; HGPRTase; HPRT1; HPRT
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种属
Human
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表达系统
E. coli
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标签
N-6*His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P00492
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表达区间
M1-A218
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氨基酸序列
MATRSPGVVISDDEPGYDLDLFCIPNHYAEDLERVFIPHGLIMDRTERLARDVMKEMGGHHIVALCVLKGGYKFFADLLDYIKALNRNSDRSIPMTVDFIRLKSYCNDQSTGDIKVIGGDDLSTLTGKNVLIVEDIIDTGKTMQTLLSLVRQYNPKMVKVASLLVKRTPRSVGYKPDFVGFEIPDKFVVGYALDYNEYFRDLNHVCVISETGKAKYKA
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蛋白长度
Full Length
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分子量
30.0 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Hypoxanthine-guanine phosphoribosyltransferase (HGPRT) is a crucial enzyme in the purine salvage pathway, which recycles purine bases for nucleotide synthesis. Deficiency in HGPRT can lead to the x-linked genetic disorder known as Lesch-Nyhan syndrome, characterized by neurological dysfunction and self-aggressive behaviors. Understanding the structure and function of HGPRT is essential for developing targeted therapies for this condition. Research on recombinant HGPRT proteins has gained traction, as these proteins can be produced in vitro for functional studies and therapeutic applications. Advances in recombinant DNA technology allow researchers to express and purify HGPRT from various host systems, such as bacteria or yeast, facilitating detailed biochemical and structural analyses. These studies aim to elucidate the enzyme's catalytic mechanisms, substrate specificity, and interactions with inhibitors. Furthermore, HGPRT's potential role as a drug target is being explored, as modulating its activity may offer novel therapeutic avenues for treating purine metabolism disorders. Overall, the investigation of recombinant HGPRT not only enhances our understanding of purine metabolism but also opens up possibilities for innovative treatments for related genetic disorders.
