Analytical Data
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基因名
DKK-3
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简介
DKK-3 protein antagonizes canonical Wnt signaling by blocking LRP5/6-Wnt interactions and forming a ternary complex with KREMEN, thereby promoting LRP5/6 internalization.In vertebrate development, DKK-3, together with other DKKs, critically inhibits Wnt-regulated processes such as axial patterning, limb development, somitogenesis, and eye formation.DKK-3 Protein, Human (HEK293, His) is the recombinant human-derived DKK-3 protein, expressed by HEK293 , with C-6*His labeled tag.
- Application
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别名
rHuDickkopf-related protein 3/DKK-3, His; Dickkopf-Related Protein 3; Dickkopf-3; Dkk-3; hDkk-3; DKK3; REIC
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种属
Human
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表达系统
HEK293
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标签
C-6*His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9UBP4
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表达区间
A22-I350
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氨基酸序列
APAPTATSAPVKPGPALSYPQEEATLNEMFREVEELMEDTQHKLRSAVEEMEAEEAAAKASSEVNLANLPPSYHNETNTDTKVGNNTIHVHREIHKITNNQTGQMVFSETVITSVGDEEGRRSHECIIDEDCGPSMYCQFASFQYTCQPCRGQRMLCTRDSECCGDQLCVWGHCTKMATRGSNGTICDNQRDCQPGLCCAFQRGLLFPVCTPLPVEGELCHDPASRLLDLITWELEPDGALDRCPCASGLLCQPHSHSLVYVCKPTFVGSRDQDGEILLPREVPDEYEVGSFMEEVRQELEDLERSLTEEMALGEPAAAAAALLGGEEI
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蛋白长度
Full Length of Mature Protein
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分子量
66-73 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
DKK-3 (Dickkopf-related protein 3) is a member of the Dickkopf family, which plays a crucial role in the regulation of the Wnt signaling pathway, important for cell proliferation, differentiation, and development. Emerging evidence suggests that DKK-3 acts as a tumor suppressor, as its expression is frequently downregulated in various malignancies, including breast, prostate, and lung cancers. Research has demonstrated that DKK-3 not only inhibits Wnt signaling but also modulates other pathways, contributing to its role in tumor progression and metastasis. Studies have shown that reintroducing DKK-3 into cancer cells can suppress their growth and increase sensitivity to chemotherapeutic agents. Additionally, DKK-3 is implicated in bone metabolism and has been linked to conditions such as osteoporosis. Therefore, understanding the molecular mechanisms by which DKK-3 functions can provide insights into its potential as a therapeutic target. Researchers are increasingly focused on developing recombinant DKK-3 proteins to explore its biological functions and therapeutic applications further. This includes studying its potential roles in inhibiting cancer cell migration and invasion while also investigating its use in regenerative medicine due to its involvement in promoting bone formation. As such, DKK-3 represents a promising candidate for targeted therapy in oncology and a valuable tool for understanding complex biological processes in development and disease.
