Analytical Data
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Gene name
PNPLA3
- Application
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Alternative Names
Acylglycerol transacylase; Adiponutrin; ADPN; Calcium-independent phospholipase A2-epsilon; iPLA2-epsilon; Lysophosphatidic acid acyltransferase; Patatin-like phospholipase domain-containing protein 3
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Species
Human
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Source
Baculovirus
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Tag
C-6*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9NST1
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Expression Region
M1-L481, I148M
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AA Sequence
MYDAERGWSLSFAGCGFLGFYHVGATRCLSEHAPHLLRDARMLFGASAGALHCVGVLSGIPLEQTLQVLSDLVRKARSRNIGIFHPSFNLSKFLRQGLCKCLPANVHQLISGKIGISLTRVSDGENVLVSDFRSKDEVVDALVCSCFMPFYSGLIPPSFRGVRYVDGGVSDNVPFIDAKTTITVSPFYGEYDICPKVKSTNFLHVDITKLSLRLCTGNLYLLSRAFVPPDLKVLGEICLRGYLDAFRFLEEKGICNRPQPGLKSSSEGMDPEVAMPSWANMSLDSSPESAALAVRLEGDELLDHLRLSILPWDESILDTLSPRLATALSEEMKDKGGYMSKICNLLPIRIMSYVMLPCTLPVESAIAIVQRLVTWLPDMPDDVLWLQWVTSQVFTRVLMCLLPASRSQMPVSSQQASPCTPEQDWPCWTPCSPKGCPAETKAEATPRSILRSSLNFFLGNKVPAGAEGLSTFPSFSLEKSL
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Protein Length
Full Length
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Molecular Weight
56.0 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PNPLA3 (patatin-like phospholipase domain-containing protein 3) has garnered significant interest in the field of metabolic liver diseases, particularly non-alcoholic fatty liver disease (NAFLD) and non-alcoholic steatohepatitis (NASH). Genetic variants, particularly the I148M polymorphism, have been strongly associated with the progression of liver disease and hepatocellular injury. This protein plays a crucial role in lipid metabolism, influencing triglyceride storage and mobilization within hepatocytes. The study of recombinant PNPLA3 proteins, particularly variants mimicking the I148M mutation, is essential for elucidating the underlying mechanisms by which these genetic alterations contribute to liver pathology. By expressing and purifying these recombinant proteins, researchers can investigate their biochemical properties, assess substrate interactions, and analyze their impact on lipid homeostasis and inflammation pathways in liver cells. Understanding the functional implications of PNPLA3 variants can lead to targeted therapeutic strategies and better stratification of patients at risk for progressive liver disease, highlighting the clinical relevance of ongoing research in this area. The insights gained from studying PNPLA3 might ultimately inform the development of novel interventions aimed at mitigating the burden of liver-related diseases globally.
