Analytical Data
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基因名
SPHK1/Sphingosine Kinase 1
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简介
The SPHK1/sphingosine kinase 1 protein is a multifunctional lipid mediator that phosphorylates sphingosine to generate sphingosine-1-phosphate (SPP). SPHK1/Sphingosine Kinase 1 Protein, Human (sf9, 363a.a) is the recombinant human-derived SPHK1/Sphingosine Kinase 1, expressed by Sf9 insect cells, with tag Free labeled tag.
- Application
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别名
SK1; sphingosine kinase 1; SPHK1; SPK 1; SPK
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种属
Human
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表达系统
Baculovirus
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标签
Tag Free
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9NYA1-1
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表达区间
M1-S363
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蛋白长度
Partial
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分子量
40.3 KDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Sphingosine Kinase 1 (SPHK1) is a pivotal enzyme in sphingolipid metabolism, responsible for phosphorylating sphingosine to form sphingosine-1-phosphate (S1P), a bioactive lipid that plays critical roles in various cellular processes such as proliferation, survival, migration, and immune responses. Dysregulation of SPHK1 and the consequent alterations in S1P levels have been implicated in numerous pathological conditions, including cancer, cardiovascular diseases, and inflammatory disorders. Given the importance of SPHK1 in these processes, recombinant SPHK1 protein has emerged as a valuable tool for studying sphingolipid signaling pathways and for developing potential therapeutic strategies. Research utilizing this recombinant protein allows for the characterization of SPHK1 enzymatic activity, substrate specificity, and its interactions with other cellular components in vitro. Furthermore, it enables the exploration of the enzyme's role in disease mechanisms and the assessment of small molecule inhibitors targeting SPHK1, which could lead to novel treatments for diseases associated with aberrant sphingolipid signaling. Thus, the study of recombinant SPHK1 not only enhances our understanding of sphingolipid biology but also contributes to the identification of therapeutic targets in various diseases.
