Analytical Data
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Gene name
CHAC1
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简介
The CHAC1 protein cleaves glutathione, inducing apoptosis via the ATF4-ATF3-DDIT3/CHOP cascade. It acts as a negative regulator of the Notch signaling pathway, promoting neurogenesis by inhibiting Notch cleavage. CHAC1 Protein, Human is the recombinant human-derived CHAC1 protein, expressed by E. coli , with C-Strep.
- Application
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Alternative Names
CHAC1; Glutathione-specific gamma-glutamylcyclotransferase 1; Gamma-GCG 1; Blocks Notch protein; Botch; Cation transport regulator-like protein 1
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Species
Human
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Source
E. coli
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Tag
C-StrepⅡ
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9BUX1
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Expression Region
K2-V222
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Protein Length
Partial
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Molecular Weight
27.2 kDa.
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CHAC1, or ChaC Glutathione Specific γ-Glutamylcyclotransferase 1, is a protein implicated in the cellular response to oxidative stress and is known for its role in glutathione metabolism. As a vital antioxidant, glutathione plays a key role in maintaining cellular redox homeostasis, and CHAC1 facilitates the degradation of glutathione, leading to an increase in reactive oxygen species (ROS) during stress conditions. This unique function positions CHAC1 as a critical player in various physiological and pathological processes, including inflammation, cancer progression, and neurodegenerative diseases. The recombinant production of CHAC1 has gained attention in research due to its potential therapeutic applications and the need to understand its functional dynamics in greater detail. Advances in recombinant protein technology have enabled the expression and purification of CHAC1, thereby allowing researchers to investigate its structure-function relationships, enzyme kinetics, and interaction with other cellular components. Furthermore, studying the recombinant CHAC1 protein provides insights into its regulatory mechanisms in the context of stress responses and how dysregulation may contribute to disease states. Overall, the exploration of CHAC1 as a recombinant protein not only advances our understanding of antioxidant defenses in cells but also opens avenues for the development of novel therapeutic strategies targeting oxidative stress-related disorders.
