Analytical Data
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Gene name
PDK4
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简介
PDK4 Protein phosphorylates PDHA1 and PDHA2, inhibiting pyruvate dehydrogenase activity. This reduces glucose utilization, enhances fat metabolism, and helps maintain blood glucose levels during fasting. PDK4 also regulates fatty acid oxidation and biosynthesis, generates reactive oxygen species, protects epithelial cells, and regulates cell proliferation through metabolic regulation. PDK4 Protein, Human (GST) is the recombinant human-derived PDK4 protein, expressed by E. coli , with N-GST labeled tag.
- Application
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Alternative Names
PDK4; [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4; mitochondrial; Pyruvate dehydrogenase kinase isoform 4
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Species
Human
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Source
E. coli
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Tag
N-GST
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q16654
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Expression Region
M1-M411
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AA Sequence
MKAARFVLRSAGSLNGAGLVPREVEHFSRYSPSPLSMKQLLDFGSENACERTSFAFLRQELPVRLANILKEIDILPTQLVNTSSVQLVKSWYIQSLMDLVEFHEKSPDDQKALSDFVDTLIKVRNRHHNVVPTMAQGIIEYKDACTVDPVTNQNLQYFLDRFYMNRISTRMLMNQHILIFSDSQTGNPSHIGSIDPNCDVVAVVQDAFECSRMLCDQYYLSSPELKLTQVNGKFPDQPIHIVYVPSHLHHMLFELFKNAMRATVEHQENQPSLTPIEVIVVLGKEDLTIKISDRGGGVPLRIIDRLFSYTYSTAPTPVMDNSRNAPLAGFGYGLPISRLYAKYFQGDLNLYSLSGYGTDAIIYLKALSSESIEKLPVFNKSAFKHYQMSSEADDWCIPSREPKNLAKEVAM
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Protein Length
Full Length
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Molecular Weight
72.9 kDa.
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PDK4 (Pyruvate Dehydrogenase Kinase 4) is a critical enzyme that plays a pivotal role in cellular metabolism, particularly in the regulation of glucose and fatty acid oxidation. As a member of the pyruvate dehydrogenase kinase family, PDK4 modulates the activity of the pyruvate dehydrogenase complex, which is essential for converting pyruvate to acetyl-CoA, a key metabolic intermediate that feeds into the tricarboxylic acid cycle. Dysregulation of PDK4 has been implicated in various metabolic disorders, including obesity, diabetes, and cancer, making it a significant target for therapeutic intervention. Research has shown that PDK4 expression is influenced by various factors, including insulin, glucagon, and nutrient availability, indicating its role in metabolic adaptation. Characterization of recombinant PDK4 protein allows for a detailed understanding of its structure, function, and regulation, thus providing insights into its potential as a biomarker or therapeutic target. The production of recombinant PDK4 facilitates kinetic studies, inhibitor screening, and development of metabolically targeted therapies. As the prevalence of metabolic diseases continues to rise globally, understanding the intricate role of PDK4 in metabolic pathways is essential for developing innovative strategies for disease management and treatment.
