Analytical Data
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Gene name
RNF14
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简介
The RNF14 protein is a critical E3 ubiquitin protein ligase that leads to activation of the RNF14-RNF25 translation quality control pathway during ribosome stalling. RNF14 is recruited by GCN1 to ubiquitinate and degrade translation factors, mainly EEF1A1/eEF1A, as well as ETF1/eRF1 and ribosomal proteins (RPL0, RPL1, RPL12, RPS13, RPS17). RNF14 Protein, Human (His) is the recombinant human-derived RNF14 protein, expressed by E. coli , with N-6*His labeled tag.
- Application
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Alternative Names
RNF14; E3 ubiquitin-protein ligase RNF14; Androgen receptor-associated protein 54; HFB30; RING finger protein 14; Triad2 protein
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Species
Human
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Source
E. coli
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Tag
N-6*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9UBS8
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Expression Region
S2-D474
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Protein Length
Partial
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Molecular Weight
55.8 KDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
RNF14 (RING Finger Protein 14) is a member of the RING finger E3 ubiquitin ligase family, playing a pivotal role in cell signaling, growth regulation, and the maintenance of protein homeostasis. Recent studies have highlighted its involvement in various cellular processes, including the modulation of inflammatory responses, the regulation of gene expression, and the control of cellular stress responses. Abnormal RNF14 expression has been implicated in several diseases, including cancer, neurodegenerative disorders, and autoimmune conditions, prompting researchers to explore its potential as a therapeutic target. The recombinant protein form of RNF14 is of particular interest for functional studies and drug development, allowing for detailed investigations into its biochemical properties, substrate specificity, and interaction with other proteins. By generating RNF14 as a recombinant protein, scientists can elucidate its role in cellular pathways and how it influences disease mechanisms, paving the way for novel therapeutic strategies aimed at manipulating its function or restoring its normal activity in disease states. Understanding the structural and functional dynamics of RNF14 through recombinant technology will also inform the development of small molecules or biologics that could effectively modulate its activity in relevant disease contexts.
