Analytical Data
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基因名
RNF14
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简介
The RNF14 protein is a critical E3 ubiquitin protein ligase that leads to activation of the RNF14-RNF25 translation quality control pathway during ribosome stalling. RNF14 is recruited by GCN1 to ubiquitinate and degrade translation factors, mainly EEF1A1/eEF1A, as well as ETF1/eRF1 and ribosomal proteins (RPL0, RPL1, RPL12, RPS13, RPS17). RNF14 Protein, Human (His) is the recombinant human-derived RNF14 protein, expressed by E. coli , with N-6*His labeled tag.
- Application
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别名
RNF14; E3 ubiquitin-protein ligase RNF14; Androgen receptor-associated protein 54; HFB30; RING finger protein 14; Triad2 protein
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种属
Human
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表达系统
E. coli
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标签
N-6*His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9UBS8
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表达区间
S2-D474
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蛋白长度
Partial
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分子量
55.8 KDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
RNF14 (RING Finger Protein 14) is a member of the RING finger E3 ubiquitin ligase family, playing a pivotal role in cell signaling, growth regulation, and the maintenance of protein homeostasis. Recent studies have highlighted its involvement in various cellular processes, including the modulation of inflammatory responses, the regulation of gene expression, and the control of cellular stress responses. Abnormal RNF14 expression has been implicated in several diseases, including cancer, neurodegenerative disorders, and autoimmune conditions, prompting researchers to explore its potential as a therapeutic target. The recombinant protein form of RNF14 is of particular interest for functional studies and drug development, allowing for detailed investigations into its biochemical properties, substrate specificity, and interaction with other proteins. By generating RNF14 as a recombinant protein, scientists can elucidate its role in cellular pathways and how it influences disease mechanisms, paving the way for novel therapeutic strategies aimed at manipulating its function or restoring its normal activity in disease states. Understanding the structural and functional dynamics of RNF14 through recombinant technology will also inform the development of small molecules or biologics that could effectively modulate its activity in relevant disease contexts.
