Analytical Data
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Gene name
MAD2L1
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简介
MAD2L1 is a critical spindle assembly checkpoint component that delays anaphase until correct chromosome alignment. During prophase, it forms a heterotetrameric complex with MAD1L1 at unattached kinetochores, recruiting O-MAD2 and aiding transformation. MAD2L1 Protein, Human (His-SUMO) is the recombinant human-derived MAD2L1 protein, expressed by E. coli , with N-SUMO, N-6*His labeled tag.
- Application
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Alternative Names
HsMAD2; MAD 2; MAD2 like 1; MAD2 mitotic arrest deficient like 1; MAD2-like protein 1; Mad2l1; MD2L1_HUMAN; Mitotic arrest deficient 2-like protein 1; Mitotic spindle assembly checkpoint protein MAD2A; REV7
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Species
Human
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Source
E. coli
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Tag
N-SUMO;N-6*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q13257-1
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Expression Region
A2-D205
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AA Sequence
ALQLSREQGITLRGSAEIVAEFFSFGINSILYQRGIYPSETFTRVQKYGLTLLVTTDLELIKYLNNVVEQLKDWLYKCSVQKLVVVISNIESGEVLERWQFDIECDKTAKDDSAPREKSQKAIQDEIRSVIRQITATVTFLPLLEVSCSFDLLIYTDKDLVVPEKWEESGPQFITNSEEVRLRSFTTTIHKVNSMVAYKIPVND
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Protein Length
Full Length of Isoform-1 Mature Protein
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Molecular Weight
39.4 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
MAD2L1, also known as MAD2, is a critical component of the spindle assembly checkpoint (SAC), a safeguard that ensures accurate chromosome segregation during cell division. Dysfunction in this process can lead to aneuploidy and tumorigenesis, making MAD2L1 a significant focus in cancer research. Studies have shown that MAD2L1 forms a complex with various proteins to detect unattached kinetochores, thereby preventing the onset of anaphase until all chromosomes are properly aligned. Overexpression or mutations in MAD2L1 have been associated with several types of cancers, indicating its pivotal role in maintaining genomic stability. Consequently, researchers have aimed to analyze MAD2L1's structure and function through recombinant protein studies, which help elucidate its interaction with other SAC components. By generating and purifying recombinant MAD2L1, scientists can investigate its biochemical properties and the effects of specific mutations, providing deeper insights into its mechanistic roles in cancer biology. This research not only enhances our understanding of cell cycle regulation but also holds potential for developing targeted therapies for cancer treatment.
